AB5896
Anti-P2X3 Receptor Antibody, pain
serum, Chemicon®
Sinónimos:
P2X3 Antibody
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About This Item
Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Productos recomendados
origen biológico
guinea pig
Nivel de calidad
forma del anticuerpo
serum
tipo de anticuerpo
primary antibodies
clon
polyclonal
reactividad de especies
human, rat, mouse
fabricante / nombre comercial
Chemicon®
técnicas
immunocytochemistry: suitable
immunohistochemistry: suitable
Nº de acceso NCBI
Nº de acceso UniProt
Condiciones de envío
dry ice
modificación del objetivo postraduccional
unmodified
Información sobre el gen
human ... P2RX3(5024)
Especificidad
P2X3 Receptor.
Inmunógeno
A 15 amino acid peptide corresponding to amino acids 383-397 from the carboxy-terminus of the rat P2X3 Receptor protein.
Control Peptide: Catalog Number AG356
Control Peptide: Catalog Number AG356
Aplicación
Immunohistochemistry: 1:1,000.
Immunocytochemistry: 1:1,000.
Optimal working dilutions must be determined by end user.
APPLICATION NOTES FOR AB5896
IMMUNOHISTOCHEMISTRY
Male Sprague-Dawley rats (b.wt. 100-150g) were anesthetized with sodium pentobarbital and perfused via the ascending aorta with: 1) 50 mL of Ca2+-free Tyrode+s solution followed by 2) a formalin-picric acid fixative (4% paraformaldehyde with 0.4% picric acid in 0.16 M phosphate buffer, pH 6.9) and 3) 10% sucrose in PBS as a cryo-protectant. Tissues were rapidly dissected out and stored overnight in 0.1 M phosphate buffer (pH 7.4) containing 10% sucrose. Slide-mounted tissue sections were incubated with blocking buffer for 1 hour at room temperature. Primary antibody was diluted in blocking buffer to the appropriate working dilution. Blocking buffer was removed and the slides were then incubated at 2-8°C for 18-24 hours with AB5896 (1:1,000). After rinsing in PBS 3 times sections were incubated for 60 minutes at room temperature with Cy3-conjugated secondary antibodies. After mounting in a mixture of PBS and glycerol (1:3) containing 0.1% p-phenylenediamine, sections were examined with a Nikon Microphot-SA epifluorescence microscope.
IMMUNOCYTOCHEMISTRYP
2X3 transfected cells were processed for indirect immunofluorescence. Media was removed and cells were gently washed 3 times with serum-free media. Following fixation procedure, cells were processed for indirect immunofluorescence as above.
Immunocytochemistry: 1:1,000.
Optimal working dilutions must be determined by end user.
APPLICATION NOTES FOR AB5896
IMMUNOHISTOCHEMISTRY
Male Sprague-Dawley rats (b.wt. 100-150g) were anesthetized with sodium pentobarbital and perfused via the ascending aorta with: 1) 50 mL of Ca2+-free Tyrode+s solution followed by 2) a formalin-picric acid fixative (4% paraformaldehyde with 0.4% picric acid in 0.16 M phosphate buffer, pH 6.9) and 3) 10% sucrose in PBS as a cryo-protectant. Tissues were rapidly dissected out and stored overnight in 0.1 M phosphate buffer (pH 7.4) containing 10% sucrose. Slide-mounted tissue sections were incubated with blocking buffer for 1 hour at room temperature. Primary antibody was diluted in blocking buffer to the appropriate working dilution. Blocking buffer was removed and the slides were then incubated at 2-8°C for 18-24 hours with AB5896 (1:1,000). After rinsing in PBS 3 times sections were incubated for 60 minutes at room temperature with Cy3-conjugated secondary antibodies. After mounting in a mixture of PBS and glycerol (1:3) containing 0.1% p-phenylenediamine, sections were examined with a Nikon Microphot-SA epifluorescence microscope.
IMMUNOCYTOCHEMISTRYP
2X3 transfected cells were processed for indirect immunofluorescence. Media was removed and cells were gently washed 3 times with serum-free media. Following fixation procedure, cells were processed for indirect immunofluorescence as above.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Neurotransmitters & Receptors
Neuroinflammation & Pain
Neurotransmitters & Receptors
Neuroinflammation & Pain
This Anti-P2X3 Receptor Antibody, pain is validated for use in IC, IH for the detection of P2X3 Receptor.
Forma física
Serum. Liquid. Contains 0.05% sodium azide.
Almacenamiento y estabilidad
Maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.
Información legal
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Cláusula de descargo de responsabilidad
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Código de clase de almacenamiento
10 - Combustible liquids
Clase de riesgo para el agua (WGK)
WGK 1
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
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Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.
Juliana Maia Teixeira et al.
Molecular neurobiology, 54(8), 6174-6186 (2016-10-07)
Osteoarthritis (OA) is a degenerative and progressive disease characterized by cartilage breakdown and by synovial membrane inflammation, which results in disability, joint swelling, and pain. The purinergic P2X3 and P2X2/3 receptors contribute to development of inflammatory hyperalgesia, participate in arthritis
Localization of P2X2 and P2X3 receptors in rat trigeminal ganglion neurons.
Staikopoulos, V; Sessle, BJ; Furness, JB; Jennings, EA
Neuroscience null
Central connectivity of transient receptor potential melastatin 8-expressing axons in the brain stem and spinal dorsal horn.
Kim, YS; Park, JH; Choi, SJ; Bae, JY; Ahn, DK; McKemy, DD; Bae, YC
Testing null
P2X(2)- and P2X(3)-positive fibers in fungiform papillae originate from the chorda tympani but not the trigeminal nerve in rats and mice.
Yusuke Ishida,Shinya Ugawa,Takashi Ueda,Takahiro Yamada,Yasuhiro Shibata,Aki Hondoh et al.
The Journal of Comparative Neurology null
Z-J Wang et al.
Anatomy and embryology, 207(4-5), 363-371 (2003-11-19)
Intraganglionic laminar endings (IGLEs) represent the most prominent vagal afferent terminal structures throughout the gastrointestinal tract. They are most prominent in the esophagus and stomach, but can be found down to the distal colon. Their role as mechanosensors as proposed
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