AB5312

Anti-Growth Associated Protein 43 Antibody

serum, Chemicon^®

• Sinónimos: GAP-43, Neuromodulin, B-50
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propiedades

• origen biológico: rabbit
• Nivel de calidad: 100
• forma del anticuerpo: serum
• tipo de anticuerpo: primary antibodies
• clon: polyclonal
• reactividad de especies: mouse, human, monkey, rat, feline, bovine
• fabricante / nombre comercial: Chemicon^®
• técnicas: immunocytochemistry: suitable; immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable; immunoprecipitation (IP): suitable; western blot: suitable
• Nº de acceso NCBI: NM_002045.2
• Nº de acceso UniProt: P17677
• Condiciones de envío: dry ice
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... GAP43(2596)

Descripción

Descripción general

GAP-43, a common marker of differentiating neurons, is expressed at elevated levels by developing or regenerating neurons during axon growth. While GAP-43 is an integral membrane protein associated with the cytoplasmic surface of axonal growth cones (and synapses), it is absent from dendritic growth cones. In adult brain, GAP-43 is found in high concentration in presynaptic areas where memory formation is thought to occur, such as frontal cortex, limbic system and hippocampus. Phosphorylation of GAP-43 by PKC (at Ser41 on human, mouse, rat, and bovine GAP-43 or Ser42 on chicken and Xenopus GAP-43) is specifically correlated with certain forms of synaptic plasticity.

Especificidad

AB5312 specifically recognizes GAP-43 regardless of the protein′s phosphorylation state. This antiserum has been refered to in the literature as CG-1.

Inmunógeno

Purified GAP-43 from feline brain (McIntosh and Parkinson, 1990).

Aplicación

Immunocytochemistry: 1:100-1:400 for mouse primary neurons, NTera2 neurons.

Immuohistochemistry: 1:2,000 on coronal sections of adult visual cortex (McIntosh and Parkinson, 1990). AB5312 has been used to stain GAP-43 in 4% paraformaldehyde-fixed paraffin-embedded tissue sections of mouse olfactory bulb (Legrier, 2001).

Western blot: 1:500 - 1:1,000. Detects a single band at approximately 50 kDa on western blots of protein extracts from adult visual cortex (McIntosh and Parkinson, 1990).

Immunoprecipitation

Optimal working dilutions must be determined by end user.

Research Category
Neuroscience

Research Sub Category
Neuroregenerative Medicine

Signaling Neuroscience

This Anti-Growth Associated Protein 43 Antibody is validated for use in IC, IH, IH(P), IP, WB for the detection of Growth Associated Protein 43.

Descripción de destino

23.6 kDa

Forma física

Serum. Liquid with 0.02% sodium azide added as a preservative.

Unpurified

Almacenamiento y estabilidad

Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Nota de análisis

Control
Rat dorsal root ganglion tissue that has been subjected to a spinal nerve ligation, cultured neurons

Otras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Información legal

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 10 - Combustible liquids
• Clase de riesgo para el agua (WGK): WGK 2
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable