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Key Documents

AB5246

Sigma-Aldrich

Anti-P2X7 Receptor Antibody

Chemicon®, from rabbit

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About This Item

Código UNSPSC:
12352203
eCl@ss:
32160702

origen biológico

rabbit

forma del anticuerpo

affinity purified immunoglobulin

tipo de anticuerpo

primary antibodies

clon

polyclonal

purificado por

affinity chromatography

reactividad de especies

rat

fabricante / nombre comercial

Chemicon®

técnicas

immunohistochemistry: suitable
western blot: suitable

Nº de acceso NCBI

Nº de acceso UniProt

Condiciones de envío

wet ice

modificación del objetivo postraduccional

unmodified

Información sobre el gen

human ... P2RX7(5027)

Especificidad

The epitope is specific for P2X7 and is not present in any other known protein.

SPECIES REACTIVITIES: Rat, mouse (Solee et al. 2001) and Candida albicans (Koshlukova et al. 2000). The sequence is highly conserved in mouse and human (18/20 and 16/20 residues identical, respectively). Reactivity with other species has not been tested.

Inmunógeno

Purified peptide P2X7 from 576-595 of rat P2X7 (Koshlukova et al. 2000).

Aplicación

Anti-P2X7 Receptor Antibody detects level of P2X7 Receptor & has been published & validated for use in IH & WB.
Research Category
Neuroscience
Research Sub Category
Neurotransmitters & Receptors
Western blot:1:200-1:1000 using ECL on rat brain membranes.

Immunohistochemistry (Vial & Evans 2000; Lewis & Evans 2000; Gitterman & Evans 2000)

Dilutions should be made using a carrier protein such as BSA (1-3%)

Optimal working dilutions must be determined by the end user.

Forma física

Affinity purified immunoglobulin. Lyophilized from phosphate buffered saline, pH 7.4, containing 1% BSA and 0.05% sodium azide as a preservative. Reconstitute with 200 μL of sterile deionized water. Centrifuge antibody preparation before use (10,000 xg for 5 min).

Almacenamiento y estabilidad

Maintain lyophilized material at -20°C for up to 12 months. After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.

Nota de análisis

Control
CONTROL ANTIGEN: Included free of charge with the antibody is 40 μg of control antigen (lyophilized powder). The stock solution of the antigen can be made up using 100 μL of sterile distilled water. For negative control, preincubate 1 μg of peptide with 1 μg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.

Otras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Información legal

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Frases de peligro

Consejos de prudencia

Clasificaciones de peligro

Aquatic Chronic 3

Código de clase de almacenamiento

11 - Combustible Solids

Clase de riesgo para el agua (WGK)

WGK 3


Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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S E Koshlukova et al.
Infection and immunity, 68(12), 6848-6856 (2000-11-18)
Salivary histatins (Hsts) are antifungal peptides with promise as therapeutic agents against candidiasis. Hst 5 kills the fungal pathogen Candida albicans via a mechanism that involves release of cellular ATP in the absence of cytolysis. Here we demonstrate that released
C Vial et al.
British journal of pharmacology, 131(7), 1489-1495 (2000-11-23)
1. We have used subtype selective P2X receptor antibodies to determine the expression of P2X(1 - 7) receptor subunits in the mouse urinary bladder. In addition we have compared P2X receptor mediated responses in normal and P2X(1) receptor deficient mice
C J Lewis et al.
British journal of pharmacology, 131(8), 1659-1666 (2001-01-05)
Immunoreactivity for P2X(1), P2X(4) and P2X(5) receptor subtypes was detected in the smooth muscle cell layer of second and third order rat mesenteric arteries immunoreactivity, for P2X(2), P2X(3), P2X(6) and P2X(7) receptors was below the level of detection in the
Theresa Puthussery et al.
The Journal of comparative neurology, 472(1), 13-23 (2004-03-17)
The distribution of P2X(7) receptor (P2X(7)R) subunits was studied in the rat retina using a subunit-specific antiserum. Punctate immunofluorescence was observed in the inner and outer plexiform layers. Double labeling of P2X(7) and the horizontal cell marker, calbindin, revealed extensive
Jonathan R Genzen et al.
Purinergic signalling, 5(3), 299-307 (2009-03-11)
Ependymal cells line the cerebral ventricles and are located in an ideal position to detect central nervous system injury and inflammation. The signaling mechanisms of ependymal cells, however, are poorly understood. As extracellular adenosine 5'-triphosphate is elevated in the context

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