AB3879
Anti-Acetyl Lysine Antibody
Chemicon®, from rabbit
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About This Item
Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Productos recomendados
origen biológico
rabbit
Nivel de calidad
forma del anticuerpo
affinity isolated antibody
tipo de anticuerpo
primary antibodies
clon
polyclonal
purificado por
affinity chromatography
reactividad de especies (predicha por homología)
all
fabricante / nombre comercial
Chemicon®
técnicas
ELISA: suitable
immunofluorescence: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
Condiciones de envío
wet ice
modificación del objetivo postraduccional
acetylation (Lys)
Descripción general
In the nucleus, DNA is tightly packed into nucleosomes generating an environment which is highly repressive towards DNA processes such as transcription. Acetylation of lysine residues within proteins has emerged as an important mechanism used by cells to overcome this repression. The acetylation of non-histone proteins such as transcription factors, as well as histones appears to be involved in this process. Acetylation may result in structural transitions as well as specific signaling within discrete chromatin domains. The role of acetylation in intracellular signaling has been inferred from the binding of acetylated peptides by the conserved bromodomain. Furthermore, recent findings suggest that bromodomain/acetylated-lysine recognition can serve as a regulatory mechanism in protein-protein interactions in numerous cellular processes such as chromatin remodeling and transcriptional activation.
Especificidad
Recognizes proteins acetylated on lysine residues. This antibody recognizes multiple acetylated proteins including acetylated histones, acetylated BSA, and acetylated MBP, and exhibits no reaction with non-acetylated proteins.
Inmunógeno
Acetylated KLH.
Aplicación
Anti-Acetyl Lysine Antibody is a high quality Rabbit Polyclonal Antibody for the detection of Acetyl Lysine & has been validated in ELISA, IF, IHC, IP & WB.
Immunoprecipitation:
A previous lot was used at 10 µg/mg crude protein.
ELISA:
0.25-1 μg/mL of a previous lot was used.
Immunohistochemistry:
2.5 μg/mL of a previous lot was used.
Immunofluorescence:
5 μg/mL of a previous lot was used.
Optimal working dilutions must be determined by end user.
A previous lot was used at 10 µg/mg crude protein.
ELISA:
0.25-1 μg/mL of a previous lot was used.
Immunohistochemistry:
2.5 μg/mL of a previous lot was used.
Immunofluorescence:
5 μg/mL of a previous lot was used.
Optimal working dilutions must be determined by end user.
Research Category
Signaling
Epigenetics & Nuclear Function
Signaling
Epigenetics & Nuclear Function
Research Sub Category
General Post-translation Modification
Histones
General Post-translation Modification
Histones
Calidad
Routinely evaluated by Western Blot on Hela Acid Extract/Sodium Butyrate lysates.
Western Blot Analysis: 1:500 dilution of this lot detected lysine acylated proteins on 10 μg of Hela Acid Extract/Sodium Butyrate lysates.
Western Blot Analysis: 1:500 dilution of this lot detected lysine acylated proteins on 10 μg of Hela Acid Extract/Sodium Butyrate lysates.
Forma física
ImmunoAffinity Purified
Purified rabbit polyclonal in buffer containing PBS with 50% glycerol.
Almacenamiento y estabilidad
Stable for 1 year at -20°C in undiluted aliquots from date of receipt.
Handling Recommendations:
Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variabillity in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
Handling Recommendations:
Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variabillity in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
Nota de análisis
Control
TSA-treated HeLa and NIH/3T3 cells, osteosarcoma tissue.
TSA-treated HeLa and NIH/3T3 cells, osteosarcoma tissue.
Otras notas
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Información legal
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Cláusula de descargo de responsabilidad
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Código de clase de almacenamiento
10 - Combustible liquids
Clase de riesgo para el agua (WGK)
WGK 2
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
¿Ya tiene este producto?
Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.
Terry R Medler et al.
Molecular cancer research : MCR, 14(10), 994-1008 (2016-07-01)
Stat5a is a transcription factor utilized by several cytokine/hormone receptor signaling pathways that promotes transcription of genes associated with proliferation, differentiation, and survival of cancer cells. However, there are currently no clinically approved therapies that directly target Stat5a, despite ample
Distinct targets of the Eco1 acetyltransferase modulate cohesion in S phase and in response to DNA damage.
Heidinger-Pauli, JM; Unal, E; Koshland, D
Molecular Cell null
Annamaria Sandomenico et al.
mAbs, 8(8), 1575-1589 (2016-10-30)
Post-translational modifications (PTMs) strongly influence the structure and function of proteins. Lysine side chain acetylation is one of the most widespread PTMs, and it plays a major role in several physiological and pathological mechanisms. Protein acetylation may be detected by
PSG gene expression is up-regulated by lysine acetylation involving histone and nonhistone proteins.
Camolotto, SA; Racca, AC; Ridano, ME; Genti-Raimondi, S; Panzetta-Dutari, GM
Testing null
Multiple histone deacetylases repress tumor suppressor gene ARHI in breast cancer.
Weiwei Feng,Zhen Lu,Robert Z Luo,Xiaohong Zhang,Edward Seto,Warren S-L Liao,Yinhua Yu
International Journal of Cancer. Journal International Du Cancer null
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