AB3831-I

Anti-phospho Cofilin-1 Antibody (Ser3)

from rabbit, purified by affinity chromatography

• Sinónimos: Cofilin-1, Ser3-phosphorylated, 18 kDa phosphoprotein, Ser3-phosphorylated, Cofilin, non-muscle isoform, Ser3-phosphorylated
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propiedades

• origen biológico: rabbit
• Nivel de calidad: 100
• forma del anticuerpo: affinity isolated antibody
• tipo de anticuerpo: primary antibodies
• clon: polyclonal
• purificado por: affinity chromatography
• reactividad de especies: human
• reactividad de especies (predicha por homología): bovine (based on 100% sequence homology), porcine (based on 100% sequence homology), mouse (based on 100% sequence homology), rat (based on 100% sequence homology)
• técnicas: inhibition assay: suitable (peptide); western blot: suitable
• Nº de acceso NCBI: NP_005498
• Nº de acceso UniProt: P23528
• Condiciones de envío: ambient
• modificación del objetivo postraduccional: phosphorylation (pSer3)
• Información sobre el gen: human ... CFL1(1072)

Descripción

Descripción general

Cofilin-1 (UniProt P23528; also known as 18 kDa phosphoprotein, Cofilin non-muscle isoform, p18) is encoded by the CFL1 (also known as CFL) gene (Gene ID 1072) in human. Cofilin-1 (non-muscle cofilin), cofilin-2 (muscle cofilin), and actin-depolymerizing factor (ADF) play an essential role in regulating actin filament dynamics and reorganization by stimulating the severance and depolymerization of actin filaments. The three cofilin family members share 70% to 81% sequence identity, exhibit similar biological functions, and display distinct but overlapping expression patterns. Cofilins and ADF bind actin monomers and filaments (G-actin and F-actin) and increase the number of actin monomers and filament fragments, facilitating filament turnover and treadmilling at key locations in migrating cells. Cofilins and ADF are inactivated by phosphorylation at Ser3 by LIM-kinases (LIMKs) and testicular protein kinases (TESKs) and are reactivated by dephosphorylation by the slingshot (SSH) family of protein phosphatases and chronophin. Cfl1-knockout results in mouse embryonic lethality at E11.5 12.5 due to aberrant neural tube closure and defective neural crest cell migration, while Adf-deficient mice are viable with normal brain appearance, but suffer from corneal defects that cause blindness in adult mice.

Especificidad

Target band detection by this polyclonal antibody was prevented by preblocking with the immunogen phosphopeptide, but not the corresponding non-phosphopeptide.

Inmunógeno

KLH-conjugated linear peptide corresponding to the N-termnal sequence of human cofilin-1 with phosphorylated Ser3.

Aplicación

Peptide Inhibition Assay: Target band detection in 10 µg of HeLa cell lysate was prevented by preblocking a representative lot with the immunogen phosphopeptide, but not the corresponding non-phosphopeptide.

Research Category
Cell Structure

This rabbit polyclonal Anti-phospho Cofilin-1 (Ser3), Cat. No. AB3831-I is validated for use in Peptide Inhibition Assay and Western Blotting for the detection of Cofilin-1 Ser3 phosphorylation.

Calidad

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: A 1:200 dilution of this antibody detected Ser3-phosphorylatd cofilin-1 in 10 µg of HeLa cell lysate.

Descripción de destino

~19 kDa observed. 18.39/18.37/184.3/18.39/18.40 kDa (bovine/human/mouse/porcine/rat) calculated (Met1 removed). Uncharacterized bands may be observed in some lysate(s).

Forma física

Affinity purified.

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Almacenamiento y estabilidad

Stable for 1 year at 2-8°C from date of receipt.

Otras notas

Concentration: Please refer to lot specific datasheet.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable