AB3795

Anti-Cortactin Antibody, phospho-specific [Tyr466]

Chemicon^®, from rabbit

• Sinónimos: Amplaxin, Oncogene EMS1
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propiedades

• origen biológico: rabbit
• Nivel de calidad: 100
• forma del anticuerpo: affinity isolated antibody
• tipo de anticuerpo: primary antibodies
• clon: polyclonal
• purificado por: affinity chromatography
• reactividad de especies: mouse
• fabricante / nombre comercial: Chemicon^®
• técnicas: western blot: suitable
• Nº de acceso NCBI: NM_005231.2; NM_138565.1
• Nº de acceso UniProt: Q14247
• Condiciones de envío: wet ice
• modificación del objetivo postraduccional: phosphorylation (pTyr466)
• Información sobre el gen: human ... CTTN(2017)

Descripción

Descripción general

Cortactin is cytoskeleton protein that facilitates assembly of cortical actin. Cortactin is widely expressed in most adherent cells and is a prominent substrate of protein-tyrosine kinase Src in vivo and in vitro. The protein sequence of cortactin contains multiple amino-terminal tandem repeats of a unique 37-amino acid sequence, which associates with actin, and a Src homology 3 domain at the carboxyl terminus.

Especificidad

The antibody detects the 80-85 kDa protein of cortactin.

Inmunógeno

Phosphorylated cortactin (Tyr466) synthetic peptide corresponding to mouse amino acid residues around tyrosine 466 (tyrosine 470 in the human sequence).

Aplicación

Research Category
Cell Structure

Research Sub Category
Cytoskeleton

This Anti-Cortactin Antibody, phospho-specific [Tyr466] is validated for use in WB for the detection of Cortactin.

Forma física

Antibody is cross-adsorbed to nonphospho-peptide corresponding to the site of phosphorylation and preadsorbed with phosphopeptides to cortactin tyrosine 421 and 482 to remove cross-reactivity then affinity purified using phosphorylated cortactin (Tyr466) peptide. Antibody is supplied in phosphate-buffered saline (no Ca²⁺, no Mg²⁺), pH 7.3, containing 1 mg/ml BSA, and 0.05% sodium azide.

Almacenamiento y estabilidad

Maintain at -20°C for up to one year from date of receipt. Avoid repeated freeze/thaw cycles.

Otras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Información legal

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 10 - Combustible liquids
• Clase de riesgo para el agua (WGK): WGK 2