AB3203

Anti-Tubulin Antibody, δ 2

Chemicon^®, from rabbit

• Sinónimos: Anti-α-tubulin, Tubulin antibody
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propiedades

• origen biológico: rabbit
• Nivel de calidad: 100
• forma del anticuerpo: affinity purified immunoglobulin
• tipo de anticuerpo: primary antibodies
• clon: polyclonal
• purificado por: affinity chromatography
• reactividad de especies: sea urchin, echinoderm, plant
• no debe reaccionar con: Saccharomyces cerevisiae
• reactividad de especies (predicha por homología): mammals
• fabricante / nombre comercial: Chemicon^®
• técnicas: immunocytochemistry: suitable; immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable; western blot: suitable
• Nº de acceso NCBI: NM_016261.2
• Nº de acceso UniProt: Q9UJT1
• Condiciones de envío: wet ice
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... TUBD1(51174)

Descripción

Descripción general

Tubulin, the dimeric subunit of microtubules, is submitted to post translational modifications including cyclic tyrosine removal and addition at the COOH terminus of the alpha subunit. The cycle involves two enzymes, TTL (Tubuline Tyrosine Ligase) and Tubulin carboxypeptidase, and generates two major forms: tyrosinated tubulin and detyrosinated tubulin (Glu-tubulin). Removal of the COOH-terminal Glu residue from the alpha chain of Glu-tubulin generates the delta2-tubulin form.

Especificidad

The antibody specifically recognizes the delta 2 form of the tubulin alpha chain (Paturle-Lafanechere et al. 1991).

SPECIES REACTIVITY:

Mammals, sea urchin, plants, not yeast (Lafanechere et al. 1994).

Inmunógeno

Peptide corresponding to the C-terminal of delta 2 tubulin.

Aplicación

Research Category
Cell Structure

Research Sub Category
Cytoskeleton

This Anti-Tubulin Antibody, δ 2 is validated for use in IH(P), WB, IC, IH for the detection of Tubulin.

Western blot: 1:1,000

Immunohistochemistry and EM on either frozen or paraffin embedded, fixed tissue (Mialhe et al. 2001): 1:500

Optimal working dilutions must be determined by the end user.

Forma física

Affinity purified immunoglobulin. Liquid in PBS.

Almacenamiento y estabilidad

Maintain at 2-8°C in undiluted for up to 6 months from date of receipt.

Otras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Información legal

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 2
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable