AB2989

Anti-Metadherin Antibody

from rabbit, purified by affinity chromatography

• Sinónimos: Astrocyte elevated gene-1 protein, Lysine-rich CEACAM1 co-isolated protein, Metastasis adhesion protein, astrocyte elevated gene-1
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41
• clon: polyclonal
• application: ICC; WB
• reactividad de especies: human, mouse
• técnicas: immunocytochemistry: suitable; western blot: suitable
• citations: 1

Propiedades

• origen biológico: rabbit
• forma del anticuerpo: affinity isolated antibody
• tipo de anticuerpo: primary antibodies
• clon: polyclonal
• purificado por: affinity chromatography
• reactividad de especies: human, mouse
• técnicas: immunocytochemistry: suitable; western blot: suitable
• isotipo: IgG
• Nº de acceso NCBI: NP_080278.3
• Nº de acceso UniProt: Q80WJ7
• Condiciones de envío: wet ice
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... MTDH(92140)

Descripción

Descripción general

Metadherin is a type 2 transmembrane protein who′s overexpression was first described in breast cancers. It plays an important role in the metastasis of breast cancers into the lungs as a secondary site of development. Antibodies reactive to the lung-homing domain of metadherin and siRNA-mediated knockdown of metadherin expression in breast cancer cells inhibited experimental lung metastasis, indicating that tumor cell metadherin mediates localization at the metastatic site (Brown, 2009).

Especificidad

The antibody recognizes Metadherin at and around the homing domain.

Inmunógeno

Epitope: Homing domain

Recombinant GST-tagged proteins corresponding at or around the lung homing domain of mouse Metadherin.

Aplicación

Anti-Metadherin Antibody is an antibody against Metadherin for use in WB & IC.

Research Category
Cell Structure

Research Sub Category
Adhesion (CAMs)

Western blot Analysis: 0.25 µg/ml of this antibody detected Metadherin in 10 µg of HEK293, HepG2, and Jurkat cell lysates.

Immunocytochemistry Analysis: 1:500 dilution from a previous lot detected Metadherin in NIH/3T3 and HeLa cells.

Calidad

Evaluated by Western blot in HeLa cell lysate.

Western blot Analysis: 0.25 µg/ml of this antibody detected Metadherin in 10 µg of HeLa cell lysate.

Descripción de destino

~ 75 kDa

Forma física

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.

Almacenamiento y estabilidad

Stable for 1 year at 2-8°C from date of receipt.

Nota de análisis

Control
HeLa cell lysate

Otras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable