AB1922
Anti-Integrin β4 Antibody
serum, Chemicon®
Sinónimos:
CD104
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About This Item
Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Productos recomendados
origen biológico
rabbit
Nivel de calidad
forma del anticuerpo
serum
tipo de anticuerpo
primary antibodies
clon
polyclonal
reactividad de especies
human, mouse, rat
fabricante / nombre comercial
Chemicon®
técnicas
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
Nº de acceso NCBI
Nº de acceso UniProt
Condiciones de envío
wet ice
modificación del objetivo postraduccional
unmodified
Información sobre el gen
human ... ITGB4(3691)
mouse ... Itgb4(192897)
rat ... Itgb4(25724)
Especificidad
Specific for beta4 integrin as determined by immunoprecipitation. The epitope recognized is on the cytoplasmic domain of the protein and is thus intracellular. No cross-reactivity to other integrin b subunits.
Inmunógeno
Synthetic peptide derived from the COOH terminal sequence (cytoplasmic domain) of the human β4 integrin subunit (Tamura et al. 1990). The sequence used is NH2-(K)GTLSTHMDQQFFQT-amide. The lysine (K) is not in the β4 sequence and was added to conjugate the peptid
Aplicación
Anti-Integrin β4 Antibody is an antibody against Integrin β4 for use in IP, IH.
Immunoprecipitation from labeled cell extracts: 5 μL of serum is sufficient to precipitate alpha6beta4 from 1-2 x 10E6 cells. In some cases immunoprecipitation from 35S methionine labeled cells results in two bands of 95 and 55 kDa of unknown origin in addition to the 200 kDa and 140 kDa bands corresponding to beta4 and alpha6.
Immunohistochemistry: 5 μg/mL. See protocol below.
Optimal working dilutions must be determined by the end user.
Suggested Protocol for Immunohistochemical:
1. Fix cryostat sections with a 1:1 chloroform-acetone mixture for 10 minutes at room temperature.
2. Air Dry.
3. Wash with 2 mL of 1x PBS.
4. Add 200 μL of first antibody (5 μg/mL) to each slide. Incubate for 1 hour at room temperature in a humidity chamber.
5. Wash with 5 mL of 1x PBS.
6. Add appropriately diluted anti-rabbit secondary antibody conjugate and incubate for 1 hour in a humidity chamber
7. Wash with 5 mL of 1x PBS.
8. Add 200 μL of development solution (containing DAB or other substrate depending on the secondary antibody) and incubate at room temperature for the necessary time to develop the dark color. Follow the reaction with a microscope, to establish the correct color intensity.
9. Block the reaction by washing with 2 mL of 1x PBS.
10. Counterstain with hematoxylin as desired.
Immunohistochemistry: 5 μg/mL. See protocol below.
Optimal working dilutions must be determined by the end user.
Suggested Protocol for Immunohistochemical:
1. Fix cryostat sections with a 1:1 chloroform-acetone mixture for 10 minutes at room temperature.
2. Air Dry.
3. Wash with 2 mL of 1x PBS.
4. Add 200 μL of first antibody (5 μg/mL) to each slide. Incubate for 1 hour at room temperature in a humidity chamber.
5. Wash with 5 mL of 1x PBS.
6. Add appropriately diluted anti-rabbit secondary antibody conjugate and incubate for 1 hour in a humidity chamber
7. Wash with 5 mL of 1x PBS.
8. Add 200 μL of development solution (containing DAB or other substrate depending on the secondary antibody) and incubate at room temperature for the necessary time to develop the dark color. Follow the reaction with a microscope, to establish the correct color intensity.
9. Block the reaction by washing with 2 mL of 1x PBS.
10. Counterstain with hematoxylin as desired.
Información legal
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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Código de clase de almacenamiento
10 - Combustible liquids
Clase de riesgo para el agua (WGK)
WGK 1
Certificados de análisis (COA)
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Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.
N Basora et al.
The Journal of biological chemistry, 274(42), 29819-29825 (1999-10-09)
Integrins are important mediators of cell-laminin interactions. In the small intestinal epithelium, which consists of spatially separated proliferative and differentiated cell populations located, respectively, in the crypt and on the villus, laminins and laminin-binding integrins are differentially expressed along the
Differential expression of integrin mRNAs and proteins during normal rat mammary gland development and in carcinogenesis.
R Y Huang, M M Ip, R Y Huang, M M Ip
Cell and Tissue Research null
Human bronchial epithelial cells secrete laminin 5, express hemidesmosomal proteins, and assemble hemidesmosomes.
P H Michelson, M Tigue, J C Jones
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society null
Upregulation of a functional form of the beta4 integrin subunit in colorectal cancers correlates with c-Myc expression.
Ni, H; Dydensborg, AB; Herring, FE; Basora, N; Gagne, D; Vachon, PH; Beaulieu, JF
Oncogene null
Modes of regulation of laminin-5 production by rat astrocytes.
S Wagner, H Gardner
Neuroscience Letters null
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