AB19015
Anti-MMP-2 Antibody, catalytic domain
Chemicon®, from rabbit
Sinónimos:
Gelatinase A, 72 kDa Type IV Collagenase
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About This Item
Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Productos recomendados
origen biológico
rabbit
Nivel de calidad
forma del anticuerpo
purified immunoglobulin
tipo de anticuerpo
primary antibodies
clon
polyclonal
reactividad de especies
human, mouse, rat
fabricante / nombre comercial
Chemicon®
técnicas
immunoprecipitation (IP): suitable
western blot: suitable
Nº de acceso NCBI
Nº de acceso UniProt
Condiciones de envío
wet ice
modificación del objetivo postraduccional
unmodified
Información sobre el gen
human ... MMP2(4313)
Especificidad
Recognizes rat MMP-2. Exhibits no cross-reactivity with other MMP family members. The antibody was generated using E. coli-expressed active rat 72 kDa type IV collagenase (catalytic domain) as an immunogen.
Inmunógeno
E. coli-expressed active rat 72 kDa type IV collagenase (catalytic domain)
Epitope: catalytic domain
Aplicación
Detect MMP-2 using this Anti-MMP-2 Antibody, catalytic domain validated for use in IP & WB.
Research Category
Cell Structure
Cell Structure
Research Sub Category
MMPs & TIMPs
MMPs & TIMPs
Western blot: 1:2,000
Immunoprecipitation: 1:500
MMP-2 [Gelatinase-A] is constitutively produced in quiescent cells and tissues, and the enzyme has a high specific activity against denatured collagen. The low protein levels produced in cell culture (pg/mL) are often below the threshold of detection by standard Western Blotting. The enzyme can be concentrated from culture media by gelatin-agarose affinity chromatography (Goldberg, 1992) or immunoprecipitation. This can prevent appearance of a spurious band of antibody binding in the vicinity of 68 kDa displayed by some concentrated media.
Optimal working dilutions must be determined by end user.
Immunoprecipitation: 1:500
MMP-2 [Gelatinase-A] is constitutively produced in quiescent cells and tissues, and the enzyme has a high specific activity against denatured collagen. The low protein levels produced in cell culture (pg/mL) are often below the threshold of detection by standard Western Blotting. The enzyme can be concentrated from culture media by gelatin-agarose affinity chromatography (Goldberg, 1992) or immunoprecipitation. This can prevent appearance of a spurious band of antibody binding in the vicinity of 68 kDa displayed by some concentrated media.
Optimal working dilutions must be determined by end user.
Ligadura / enlace
Replaces: 04-1048
Forma física
Format: Purified
Purified immunoglobulin. Liquid in PBS, containing 0.05% sodium azide.
Almacenamiento y estabilidad
Maintain refrigerated at 2-8°C in undiluted aliquots for up to 12 months.
Otras notas
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Información legal
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Cláusula de descargo de responsabilidad
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Código de clase de almacenamiento
10 - Combustible liquids
Clase de riesgo para el agua (WGK)
WGK 2
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
¿Ya tiene este producto?
Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.
Merry L Lindsey et al.
Cardiovascular research, 66(2), 410-419 (2005-04-12)
To evaluate the effects of aging on left ventricular (LV) geometry, collagen levels, matrix metalloproteinase (MMP) and tissue inhibitor of metalloproteinase (TIMP) abundance, and myocardial fibroblast function. Young (3-month-old; n=28), middle-aged (MA; 15-month-old; n=17), and old (23-month-old; n=16) CB6F1 mice
Preconditioning decreases ischemia/reperfusion-induced release and activation of matrix metalloproteinase-2.
Manoj M Lalu,Csaba Csonka,Zoltan Giricz,Tamas Csont,Richard Schulz,Peter Ferdinandy
Biochemical and biophysical research communications null
Jennifer A Kwan et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 18(6), 690-692 (2004-02-10)
Matrix metalloproteinases (MMPs) are traditionally known for their role in extracellular matrix remodeling. Increasing evidence reveals several alternative substrates and novel biological roles for these proteases. Recent evidence showed the intracellular localization of MMP-2 within cardiac myocytes, colocalized with troponin
Preconditioning with chronic cerebral hypoperfusion reduces a focal cerebral ischemic injury and increases apurinic/apyrimidinic endonuclease/redox factor-1 and matrix metalloproteinase-2 expression.
Sun-Ah Choi,Eun Hee Kim,Jong Yun Lee,Hyo Suck Nam,Seo Hyun Kim,Gyung Whan Kim et al.
Current neurovascular research null
Vascular cell adhesion molecule 1 (VCAM-1) activation of endothelial cell matrix metalloproteinases: role of reactive oxygen species.
Deem, TL; Cook-Mills, JM
Blood null
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