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AB1590P

Sigma-Aldrich

Anti-Adenosine A3 Receptor Antibody, extracellular (a.a. 154-168)

Chemicon®, from rabbit

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About This Item

Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

origen biológico

rabbit

Nivel de calidad

forma del anticuerpo

affinity purified immunoglobulin

tipo de anticuerpo

primary antibodies

clon

polyclonal

purificado por

affinity chromatography

reactividad de especies

rat

fabricante / nombre comercial

Chemicon®

técnicas

ELISA: suitable
immunocytochemistry: suitable
western blot: suitable

Nº de acceso UniProt

Condiciones de envío

dry ice

modificación del objetivo postraduccional

unmodified

Información sobre el gen

human ... ADORA3(140)

Especificidad

Specific for Adenosine A3 receptor. Based on mRNA studies, the A3R has been found in rat testis and lung, kidneys, heart and brain. Other studies have found the mRNA to be highest in lung, spleen and pineal glands and moderate levels in brain kidney and testis.

Inmunógeno

Amino acid sequence corresponding to the 3rd extracellular domain (15 amino acids) of the rat A3R gene.
Epitope: extracellular (a.a. 154-168)

Aplicación

Anti-Adenosine A3 Receptor Antibody, extracellular (a.a. 154-168) is an antibody against Adenosine A3 Receptor for use in ELISA, IC & WB.
Research Category
Neuroscience
Research Sub Category
Neurotransmitters & Receptors

Circadian Rhythm & Sleep
Western blot: 1-10 micrograms per mL: A3 receptors are different sizes in different species & tissues; A3R from rat brain membranes runs as a 40kDa band, 52kDa in rabbit uroepithelium membranes (Yu, W et al. Am J. Physiol Cell Physiol 2006 in press); use proteinase inhibitors and membrane preparations for best signals.

ELISA: 1:10,000-1:50,000 (50-100 ng A3R peptide (Catalog Number AG294)/well)

Immunocytochemistry: Caufield, 2003. Light 2% PFA fixation 5-10 minutes --long fixations lower staining intensity.

Optimal working dilutions must be determined by end user.

Forma física

Affinity purified immunoglobulin. Liquid in PBS with 0.1% BSA

Almacenamiento y estabilidad

Maintain at -20°C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles.

Otras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Información legal

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Código de clase de almacenamiento

10 - Combustible liquids

Clase de riesgo para el agua (WGK)

WGK 2

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable


Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Adenosine receptor expression and function in bladder uroepithelium
Yu, Weiqun, et al
American Journal of Physiology. Cell Physiology, 291, C254-C265 (2006)
Different roles of adenosine A1, A2A and A3 receptors in controlling kainate-induced toxicity in cortical cultured neurons
Rebola, N.
Neurochemistry International, 47(5), 317-325 (2005)
A L Tucker et al.
Cardiovascular research, 27(1), 62-67 (1993-01-01)
Four subtypes of adenosine receptors have recently been cloned from thyroid, brain and testis. In this review we have summarised properties of these purinergic receptors. The cloned A1 and A2 subtypes are probably similar or identical to receptors that exist
N K Puffinbarger et al.
Molecular pharmacology, 47(6), 1126-1132 (1995-06-01)
A polyclonal antibody to the human adenosine A2b receptor (A2bR) was produced by immunizing a chicken with a multiple antigenic peptide consisting of eight copies of a 16-amino acid peptide, corresponding to the presumed second extracellular loop of the A2bR
Neus Garcia et al.
Journal of anatomy, 225(1), 109-117 (2014-04-24)
To date, four subtypes of adenosine receptors have been cloned (A(1)R, A(2A)R, A(2B)R, and A(3)R). In a previous study we used confocal immunocytochemistry to identify A(1)R and A(2A)R receptors at mouse neuromuscular junctions (NMJs). The data shows that these receptors

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