AB1557

Anti-NMDAR2B Antibody, pain

serum, Chemicon^®

• Sinónimos: N-methyl D-aspartate Receptor Subtype 2B, NR2B
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propiedades

• origen biológico: rabbit
• Nivel de calidad: 100
• forma del anticuerpo: serum
• tipo de anticuerpo: primary antibodies
• clon: polyclonal
• reactividad de especies: mouse, human, rat
• fabricante / nombre comercial: Chemicon^®
• técnicas: immunoprecipitation (IP): suitable; western blot: suitable
• idoneidad: not suitable for immunohistochemistry
• Nº de acceso NCBI: NM_000834.2
• Nº de acceso UniProt: Q13224
• Condiciones de envío: dry ice
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... GRIN2B(2904)

Descripción

Especificidad

Recognizes NMDAR2B. By Western blot it recognizes a 180 kDa band in rat brain membranes. Also recognizes a 70 kDa band (thought to be a contaminant). No reactivity to NMDAR2A or NMDAR2C by Western blot. Immunolabeling is blocked by preadsorption of AB1557 with the C-terminal fusion protein that was used to generate the antibody.

Inmunógeno

C-Terminal Fusion Protein of the NMDAR2B (Amino acids 984-1104).

Aplicación

Anti-NMDAR2B Antibody, pain detects level of NMDAR2B & has been published & validated for use in IP & WB.

Research Category
Neuroscience

Research Sub Category
Neurotransmitters & Receptors

Western blotting 1:200-1:1,000 (NMDAR2B is present in high concentrations in the hippocampus)


Immunoprecipitation using solubilized hippocampal slices. 3 μL will (under appropriate conditions) quantitatively immunoprecipitate all NMDAR2B in 200 μg of rat brain lysate.


Not suggested for use in immunohistochemistry.


Note: Above dilutions are with 35S-protein A; with ECL dilutions may need to be considerably higher to obtain specific immunolabeling.


Optimal working dilutions must be determined by the end user.

Descripción de destino

180 kDa

Forma física

Rabbit antiSerum. Liquid, no preservatives.

Unpurified

Almacenamiento y estabilidad

Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Nota de análisis

Control
Brain tissue, Rat Purkinje cell dendrites, rat hippocampus lysate, HEK 293 cells expressing NR2B

Otras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Información legal

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 10 - Combustible liquids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable