AB1513P
Anti-Brain Derived Neurotrophic Factor Antibody
Chemicon®, from sheep
Sinónimos:
BDNF
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About This Item
Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Productos recomendados
origen biológico
sheep
Nivel de calidad
forma del anticuerpo
purified immunoglobulin
tipo de anticuerpo
primary antibodies
clon
polyclonal
reactividad de especies
human, rat, chicken
fabricante / nombre comercial
Chemicon®
técnicas
ELISA: suitable
immunohistochemistry: suitable
western blot: suitable
Nº de acceso NCBI
Nº de acceso UniProt
Condiciones de envío
dry ice
modificación del objetivo postraduccional
unmodified
Información sobre el gen
human ... BDNF(627)
Especificidad
Recognizes Brain Derived Neurotrophic Factor (BDNF). Neutralizes BDNF, but not other neurotrophins. By one site ELISA, less than 1% cross-reactivity against mouse NGF, recombinant human NT3 or neurotrophin 4. The antibody was tested on human and rat derived samples, but based on sequence similarity it is expected to react with mouse and other mammalian species. The peptide sequences of human, rat and mouse BDNF are identical.
Inmunógeno
Recombinant Human Brain Derived Neurotrophic Factor (BDNF).
Aplicación
Anti-Brain Derived Neurotrophic Factor Antibody detects level of Brain Derived Neurotrophic Factor & has been published & validated for use in ELISA, IH, FUNC & WB.
Immunohistochemistry: 1-5 μg/mL (see suggested protocol).
Immunoblotting: 1-5 μg/mL we recommend preparing the BDNF samples for western blot by the method of Semba-Katoh, R et.al (J. Neurochem. 69(1):34-42, 1997) because BDNF is not easily extracted in standard buffers. Dissected tissues should be homogenized in 10 volumes of 100mM phosphate buffer containing 1mM EDTA, 2M guanidine hydrochloride (pH7.2), and three protease inhibitors, 10mM N-ethylmaleimide, 0.36mM pepstatin, and 1mM PMSF. Homogenates are then sonicated and centrifuged at 46K x g for 30 minutes at 4°C. BDNF migrates as ~12-18kDa monomer, with a 28-30kDa homodimer in most samples.
ELISA: 1-5 μg/mL
Inhibition of biological activity in vitro: Complete inhibition of BDNF activity on embryonic chick sensory neurons occurs at 1-5 μg/mL. Biological inhibition protocols can be found in Finn et al. (J. Neurocytol. 15:169-176, 1986).
BIOLOGICAL ACTIVITY: Neutralizes BDNF, but not other neurotrophins.
Use neat for in vivo studies.
Optimal working dilutions must be determined by end user.
Immunoblotting: 1-5 μg/mL we recommend preparing the BDNF samples for western blot by the method of Semba-Katoh, R et.al (J. Neurochem. 69(1):34-42, 1997) because BDNF is not easily extracted in standard buffers. Dissected tissues should be homogenized in 10 volumes of 100mM phosphate buffer containing 1mM EDTA, 2M guanidine hydrochloride (pH7.2), and three protease inhibitors, 10mM N-ethylmaleimide, 0.36mM pepstatin, and 1mM PMSF. Homogenates are then sonicated and centrifuged at 46K x g for 30 minutes at 4°C. BDNF migrates as ~12-18kDa monomer, with a 28-30kDa homodimer in most samples.
ELISA: 1-5 μg/mL
Inhibition of biological activity in vitro: Complete inhibition of BDNF activity on embryonic chick sensory neurons occurs at 1-5 μg/mL. Biological inhibition protocols can be found in Finn et al. (J. Neurocytol. 15:169-176, 1986).
BIOLOGICAL ACTIVITY: Neutralizes BDNF, but not other neurotrophins.
Use neat for in vivo studies.
Optimal working dilutions must be determined by end user.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Neurochemistry & Neurotrophins
Neuroinflammation & Pain
Neurochemistry & Neurotrophins
Neuroinflammation & Pain
Descripción de destino
~12-18kDa monomer, with a 28-30kDa homodimer in most samples.
Forma física
Format: Purified
Protein G Purified
Protein G Purified immunoglobulin. Lyophilized from PBS, no preservatives. Reconstitute with 500 μL of sterile distilled water.
Almacenamiento y estabilidad
Maintain lyophilized material at -20 to -70°C for up to 12 months after date of receipt. After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Glycerol (1:1) can be added for additional stability. Avoid repeated freeze/thaw cycles.
Nota de análisis
Control
Brain tissue
Brain tissue
Otras notas
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Información legal
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Cláusula de descargo de responsabilidad
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Opcional
Referencia del producto
Descripción
Precios
Código de clase de almacenamiento
13 - Non Combustible Solids
Clase de riesgo para el agua (WGK)
WGK 3
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
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Brain-derived neurotrophic factor (BDNF)-like immunoreactivity localization in the retina and brain of Cichlasoma dimerus (Teleostei, Perciformes).
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Mitchell, DJ; Blasier, KR; Jeffery, ED; Ross, MW; Pullikuth, AK; Suo, D; Park, J; Smiley et al.
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Brain-derived neurotrophic factor augments peristalsis by augmenting 5-HT and calcitonin gene-related peptide release.
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Upregulation of CREB-mediated transcription enhances both short- and long-term memory.
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