69041
B834(DE3) Competent Cells - Novagen
Escherichia coli, rod shaped
Sinónimos:
Competent cells
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About This Item
Código UNSPSC:
41106202
NACRES:
NA.81
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product name
B834(DE3) Competent Cells - Novagen, B834 is the parental strain for BL21. These hosts are methionine auxotrophs and allow high specific activity labeling of target proteins with 35S-methionine and selenomethionine for crystallography.
origen biológico
Escherichia coli
fabricante / nombre comercial
Novagen®
condiciones de almacenamiento
OK to freeze
avoid repeated freeze/thaw cycles
modo de crecimiento
adherent or suspension
morfología
rod shaped
técnicas
microbiological culture: suitable
transformación celular
transformation efficiency: >2×106 cfu/μg
Condiciones de envío
dry ice
temp. de almacenamiento
−70°C
Descripción general
B834 is the parental strain for BL21 (1). These protease-deficient hosts are methionine auxotrophs and allow high specific-activity labeling of target proteins with 35S-methionine and selenomethionine for crystallography (2).
DE3 indicates that the host is a lysogen of λDE3, and therefore carries a chromosomal copy of the T7 RNA polymerase gene under control of the lacUV5 promoter. Such strains are suitable for production of protein from target genes cloned in pET vectors by induction with IPTG.
DE3 indicates that the host is a lysogen of λDE3, and therefore carries a chromosomal copy of the T7 RNA polymerase gene under control of the lacUV5 promoter. Such strains are suitable for production of protein from target genes cloned in pET vectors by induction with IPTG.
Genotype: F-ompT hsdSB(rB- mB-) gal dcm met (DE3)
This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges us to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges us to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
B834 is the parental strain for BL21. These hosts are methionine auxotrophs and allow high specific activity labeling of target proteins with 35S-methionine and selenomethionine for crystallography.
Componentes
0.4 ml1 mlComponent
•2 × 0.2 ml5 × 0.2 mlB834(DE3)Competent Cells
•2 × 2 ml4 × 2 mlSOC Medium
•2 ng2 ngTest Plasmid
•2 × 0.2 ml5 × 0.2 mlB834(DE3)Competent Cells
•2 × 2 ml4 × 2 mlSOC Medium
•2 ng2 ngTest Plasmid
Advertencia
Toxicity: Multiple Toxicity Values, refer to MSDS (O)
Otras notas
1. Wood, W. B. (1966) J. Mol. Biol. 16, 118–133.
2. Leahy, D. J., Hendrickson, W. A., Aukhil, I., and Erickson, H. P. (1992) Science 258, 987–991.
2. Leahy, D. J., Hendrickson, W. A., Aukhil, I., and Erickson, H. P. (1992) Science 258, 987–991.
Información legal
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany
Código de clase de almacenamiento
10 - Combustible liquids
Clase de riesgo para el agua (WGK)
WGK 2
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
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