688225

Caspase-1 Substrate VI, Fluorogenic

Fluorogenic caspase-1 substrate.

• Sinónimos: Caspase-1 Substrate VI, Fluorogenic, Z-YVAD-AFC
• Fórmula empírica (notación de Hill): C₃₃H₃₆F₃N₅O₁₀
• Número de CAS: 219137-85-6
• Peso molecular: 719.66
• Número MDL: MFCD01318881
• Código UNSPSC: 12352200
• NACRES: NA.77

Propiedades

• Nivel de calidad: 100
• Ensayo: 98% (HPLC)
• Formulario: solid
• fabricante / nombre comercial: Calbiochem^®
• condiciones de almacenamiento: OK to freeze; desiccated; protect from light
• color: white to off-white
• solubilidad: DMSO: 5 mg/mL
• fluorescencia: λ_ex ~400 nm; λ_em ~505 nm
• Condiciones de envío: ambient
• temp. de almacenamiento: −20°C
• cadena SMILES: FC(F)(F)c1c2c([o][c](c1)=O)cc(cc2)NC(=O)[C@@H](NC(=O)[C@@H](NC(=O)[C@@H](NC(=O)[C@@H](NC(=O)C)Cc3ccc(cc3)O)C(C)C)C)CC(=O)O
• InChI: 1S/C33H36F3N5O10/c1-15(2)28(41-31(49)23(38-17(4)42)11-18-5-8-20(43)9-6-18)32(50)37-16(3)29(47)40-24(14-26(44)45)30(48)39-19-7-10-21-22(33(34,35)36)13-27(46)51-25(21)12-19/h5-10,12-13,15-16,23-24,28,43H,11,14H2,1-4H3,(H,37,50)(H,38,42)(H,39,48)(H,40,47)(H,41,49)(H,44,45)/t16-,23-,24-,28-/m0/s1
• Clave InChI: GWKJISJKNKIJNP-DEQDFGQZSA-N

Descripción

Descripción general

Fluorogenic ICE substrate. Reaction can be monitored by a blue to green shift in fluorescence upon cleavage of the AFC fluorophore. AFC (7-amino-4-trifluoromethylcoumarin) based fluorometric substrates are superior to AMC (7-amino-4-methylcoumarin) substrates because released AFC has a greater Stoke′s shift than AMC. Excitation/emission maxima (nm) for AFC and AMC are 400/505 and 380/460, respectively.

Fluorogenic caspase-1 substrate. Reaction can be monitored visually or quantitatively by a blue to green shift in fluorescence upon cleavage of the AFC fluorophore.

Acciones bioquímicas o fisiológicas

Cell permeable: no

Primary Target
Fluorogenic caspase-1 substrate

Product does not compete with ATP.

Reversible: no

Advertencia

Toxicity: Standard Handling (A)

Secuencia

Ac-Tyr-Val-Ala-Asp-AFC

Reconstitución

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Otras notas

Jones, R.A., et al. 1998. Hepatology 27, 1632.
Thornberry, N.A., and Lazebnik, Y. 1998. Science 281, 1312.
Walker, N.P., et al. 1994. Cell 78, 343.
Thornberry, N.A., et al. 1992. Nature 356, 768.

Información legal

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Información de seguridad

• Código de clase de almacenamiento: 11 - Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable