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Sigma-Aldrich

Protease Inhibitor Cocktail Set I

A cocktail of five protease inhibitors that will inhibit a broad range of proteases and esterases. Supplied with a data sheet.

Sinónimos:

Protease Inhibitor Mix, Protease inhibitor

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About This Item

Código UNSPSC:
12352200

formulario

lyophilized

fabricante / nombre comercial

Calbiochem®

condiciones de almacenamiento

OK to freeze
desiccated (hygroscopic)

Condiciones de envío

wet ice

temp. de almacenamiento

−20°C

Descripción general

Protease Inhibitor Cocktail Set I A is specially formulated containing five protease inhibitors that will inhibit a broad range of proteases and esterases.

Especificidad

Inhibits a broad range of proteases and esterases.

Aplicación

Protease Inhibitor Cocktail Set I – Calbiochem has been used in lysis buffer to lyse cells and perform western blotting.
Protease Inhibitor Cocktail Set I have been used as a supplement in:
  • cold radioimmunoprecipitation assay (RIPA) buffer to prepare cell lysates for western blotting
  • RIPA lysis buffer to extract total protein from rat liver tissues and AML12 cells
  • sucrose solution to homogenize mice cortices for myelin isolation

Acciones bioquímicas o fisiológicas

Protease inhibitor cocktails and specific protease inhibitors are essential tools utilized by scientists in various proteomic studies. These studies encompass protein expression, characterization, biomarker discovery, mapping post-translational modifications, protein expression profiling, and quantitative protein measurement. The application of a protease inhibitor cocktail or protease inhibitors guarantees the preservation of protein integrity, facilitating downstream analysis and characterization.

Características y beneficios

  • Protease Inhibitor Cocktails offer protection of target protein against degradation in crude extracts
  • Ready for reconstitution

Advertencia

Toxicity: Harmful (C)

Reconstitución

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 1 month at -20°C.
Reconstitute each vial with 1 ml dH2O to yield a 100X stock solution.

Otras notas

The presence of EDTA may interfere with the purification of His•Tag fusion proteins if a cell extract is applied directly to IMAC resins.

Información legal

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Pictogramas

Exclamation mark

Palabra de señalización

Warning

Frases de peligro

Clasificaciones de peligro

Eye Irrit. 2

Código de clase de almacenamiento

13 - Non Combustible Solids

Clase de riesgo para el agua (WGK)

WGK 2

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable


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