5.09290

G 418 Sulfate Salt

OmniPur^® Grade, >=730 µg/mg

• Sinónimos: OmniPur^® G418 Sulfate, Cell Culture Tested, NMD Inhibitor I
• Fórmula empírica (notación de Hill): C₂₀H₄₀N₄O₁₀ · 2H₂SO₄
• Número de CAS: 108321-42-2
• Peso molecular: 692.71
• Número MDL: MFCD00058314
• Código UNSPSC: 12352200

Propiedades

• Nivel de calidad: 200
• Ensayo: ≥90% (HPLC)
• Formulario: powder
• fabricante / nombre comercial: Calbiochem^®
• condiciones de almacenamiento: OK to freeze
• color: white
• trazas de catión: heavy metals: ≤100 ppm
• temp. de almacenamiento: 10-30°C
• InChI: 1S/C20H40N4O10.2H2O4S/c1-6(25)14-11(27)10(26)9(23)18(32-14)33-15-7(21)4-8(22)16(12(15)28)34-19-13(29)17(24-3)20(2,30)5-31-19;2*1-5(2,3)4/h6-19,24-30H,4-5,21-23H2,1-3H3;2*(H2,1,2,3,4)/t6?,7-,8+,9+,10+,11-,12-,13-,14+,15+,16-,17-,18+,19-,20+;;/m0../s1
• Clave InChI: UHEPSJJJMTWUCP-NKCAIAFTSA-N

Descripción

Descripción general

Aminoglycoside antibiotic related to gentamycin that inhibits prokaryotic and eukaryotic protein synthesis, is toxic to bacteria, yeast, protozoans, helminths, higher plant and mammalian cells. Used in molecular genetics as a selective agent for the bacterial neo^r/kan^r genes. The product of these genes, aminoglycoside 3′-phosphotransferase, inactivates G418, neomycin, and kanamycin by phosphorylation. Introduction of either of these genes into cells can confer resistance to G418, which enables cells to grow in media containing G418.

Aminoglycoside related to gentamycin that inhibits prokaryotic and eukaryotic protein synthesis. Toxic to bacteria, yeast, protozoans, helminths, higher plant, and mammalian cells. Widely used in the selection of eukaryotic expression vectors carrying the bacterial neo^r/kan^r genes. The product of these genes, aminoglycoside 3′-phosphotransferase, inactivates G418, neomycin, and kanamycin by phosphorylation. Introduction of either of these genes into cells can confer resistance to G418, which enables cells to grow in media containing G418.

Acciones bioquímicas o fisiológicas

Assay: ≥730 µg/mg

Advertencia

Toxicity: Standard Handling (A)

Reconstitución

Solubilization in H₂O at 100 mg/ml yields a clear, colorless solution.

Otras notas

Ethier, S.P., and Taback, E. 1993. Cancer Lett.74, 189.
Santerre, R.F., et al. 1991. Methods Mol. Biol.7, 245.
Maniatis, T., et al. 1989. In Molecular Cloning, A Laboratory Manual, Second Edition, Cold Spring Harbor, NY.
Edwards, S.A., and Adamson, E.D. 1987. J. Cell Physiol.133, 46.
Ernst, J.F., and Chan, R.K. 1985. J. Bacteriol.163, 8.
Canaani, D., and Berg, P. 1982. Proc. Natl. Acad. Sci. USA79, 5166.
Hirth, K.P., et al. 1982. Proc. Natl. Acad. Sci. USA79, 7356.
Ursic, D., et al. 1981. Biochem. Biophys. Res. Commun.101, 1031.
Jimenez, A., and Davies, J. 1980. Nature287, 869.

Información legal

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

OMNIPUR is a registered trademark of Merck KGaA, Darmstadt, Germany

Información de seguridad

• Pictogramas: GHS08
• Palabra de señalización: Danger
• Frases de peligro: H317 - H334
• Consejos de prudencia: P261 - P272 - P280 - P284 - P302 + P352 - P304 + P340 + P312
• Clasificaciones de peligro: Resp. Sens. 1 - Skin Sens. 1
• Código de clase de almacenamiento: 13 - Non Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 3