5.05224

Necrostatin-1

InSolution, ≥95%, 25 mM in DMSO, required for the biosynthesis of sialic acid-containing complex carbohydrates

• Sinónimos: InSolution Necrostatin-1, 5-((1H-Indol-3-yl)methyl)-3-methyl-2-thioxoimidazolidin-4-one; 5-(Indol-3-ylmethyl)-(2-thio-3-methyl)hydantoin, MTH-Trp, Methyl-thiohydantoin-tryptophan, Nec-1, Nec1, Necrosome Inhibitor I, RIP1 Inhibitor I, RIPK 1 Inhibitor I, Receptor-Interacting Protei, Necrosis Inhibitor II, 5-((1H-Indol-3-yl)methyl)-3-methyl-2-thioxoimidazolidin-4-one; 5-(Indol-3-ylmethyl)-(2-thio-3-methyl)hydantoin, MTH-Trp, Methyl-thiohydantoin-tryptophan, Nec-1, Nec1, Necrosome Inhibitor I, RIP1 Inhibitor I, RIPK 1 Inhibitor I, Receptor-Interacting Protein 1 Inhibitor I
• Fórmula empírica (notación de Hill): C₁₃H₁₃N₃OS
• Peso molecular: 259.33
• Código UNSPSC: 12352200
• NACRES: NA.77

Propiedades

• Nivel de calidad: 100
• Ensayo: ≥99% (HPLC)
• Formulario: liquid
• fabricante / nombre comercial: Calbiochem^®
• condiciones de almacenamiento: OK to freeze; avoid repeated freeze/thaw cycles; desiccated (hygroscopic); protect from light
• temp. de almacenamiento: −70°C

Descripción

Descripción general

A cell-permeable, potent, and selective blocker of necroptosis (EC₅₀ = 494 nM in FADD-deficient Jurkat cells treated with TNF-α), a nonapoptotic necrotic cell death pathway mediated by death-domain receptors (DRs) that offers neuroprotection in a murine model of ischemic brain injury. Exhibits no effect on DR-induced apoptosis. Also acts as a selective and ATP-competitive inhibitor of RIP1 kinase with negligible effect on RIP2 kinase activity. Nec-1 target appears to be a critical common necroptotic step upstream of execution events and downstream of DRs. Inactive control, Nec-1i, is also available (>Cat. No. 480066).

Acciones bioquímicas o fisiológicas

Cell permeable: yes

Reversible: yes

Envase

Packaged under inert gas

Advertencia

Toxicity: Irritant (B)

Forma física

A 25 mM (10 mg/1.54 ml) sterile-filtered solution of Necrostatin-1 (Cat. No. 480065) in DMSO.

Reconstitución

Following initial thaw, aliquot and freeze (-70°C). Aliquots are stable for up to 1 year at -70°C.

Otras notas

Degterev, A., et al. 2013. Nat. Chem. Biol.9, 192.
Degterev, A., et al. 2012. Cell Death Differ.20, 366.
Christofferson, D.E., et al. 2012. Cell Death Dis.3, e320.
Takahashi, N., et al. 2012. Cell Death Dis.3, e437.
Degterev, A., et al. 2008. Nat. Chem. Biol.4, 313.
Degterev, A., et al. 2005. Nat. Chem. Biol.1, 112.
Muller, A.J., et al. 2005. Nat. Med.11, 312.
Teng, X., et al. 2005. Bioorg. Med. Chem. Lett.15, 5039.

Información legal

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Información de seguridad

• Código de clase de almacenamiento: 10 - Combustible liquids
• Clase de riesgo para el agua (WGK): WGK 2
• Punto de inflamabilidad (°F): 188.6 °F - (refers to pure substance)
• Punto de inflamabilidad (°C): 87 °C - (refers to pure substance)