5.00583

(+)-Brefeldin A, Eupenicillium brefeldianum

InSolution, ≥95%, 25 mM in DMSO

• Sinónimos: InSolution (+)-Brefeldin A, Eupenicillium brefeldianum, 1,6,7,8,9,11a,12,13,14,14a-Decahydro-1,13-dihydroxy-6-methyl-4H-cyclopent[f]oxacyclotridecin-4-one, (1 S,2 E,7 S,10 E,12 R,13 R,15 S)-12,15-dihydroxy-7-methyl-8-oxabicyclo[11.3.0]hexadeca-2,10-dien-9-one,
• Fórmula empírica (notación de Hill): C₁₆H₂₄O₄
• Número de CAS: 20350-15-6
• Peso molecular: 280.36
• Número MDL: MFCD12913297
• Código UNSPSC: 12352200
• NACRES: NA.77

Propiedades

• Nivel de calidad: 100
• Análisis: ≥95% (HPLC)
• formulario: liquid
• fabricante / nombre comercial: Calbiochem^®
• condiciones de almacenamiento: OK to freeze; avoid repeated freeze/thaw cycles; desiccated (hygroscopic); protect from light
• temp. de almacenamiento: −70°C
• InChI: 1S/C16H24O4/c1-11-5-3-2-4-6-12-9-13(17)10-14(12)15(18)7-8-16(19)20-11/h4,6-8,11-15,17-18H,2-3,5,9-10H2,1H3/b6-4+,8-7+/t11-,12+,13-,14+,15-/m0/s1
• Clave InChI: KQNZDYYTLMIZCT-PNFJWZTBSA-N

Descripción

Descripción general

Specifically and reversibly blocks translocation of proteins from the endoplasmic reticulum (ER) to the Golgi apparatus without affecting endocytosis or lysosome function. Inhibitor of HDL-mediated cholesterol efflux. Causes disassembly of the Golgi complex and ER swelling in a variety of mammalian cell lines at <40 ng/ml. Inhibits the activity of BIG1 and BIG2, the guanine-nucleotide exchange proteins for ADP-ribosylation factors. Blocks binding of ADP-ribosylation factor to the Golgi and inhibits the GDP-GTP exchange.

Acciones bioquímicas o fisiológicas

Cell permeable: yes

Primary Target
ADP-ribosylation factor (ARF)

Reversible: yes

Envase

Packaged under inert gas

Advertencia

Toxicity: Irritant (B)

Forma física

A 25 mM (2 mg/285 µL) solution of (+)-Brefeldin A (Cat. No. 203729) in DMSO.

Reconstitución

Following initial thaw, aliquot and freeze (-70°C). Aliquots are stable for up to 6 months at -70°C. Use only fresh DMSO for further dilutions prior to use.

Otras notas

Yamagi, R., et al. 2000. Proc. Natl. Acad. Sci. USA97, 2567.
Moriango, N., et al. 1996. Proc. Natl. Acad. Sci. USA93, 12856.
Mendez, A.J. 1995. J. Biol. Chem.270, 5891.
Ridgway, N.D., et al. 1995. J. Biol. Chem. 270, 8023.
Tsai, S.C., et al. 1994. Proc. Natl. Acad. Sci. USA91, 3063.
Ktistakis, N.Y., et al. 1992. Nature356, 344.

Información legal

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Información de seguridad

• Código de clase de almacenamiento: 10 - Combustible liquids
• Clase de riesgo para el agua (WGK): WGK 2
• Punto de inflamabilidad (°F): 188.6 °F - (refers to pure substance)
• Punto de inflamabilidad (°C): 87 °C - (refers to pure substance)