499050

Osteocalcin, Bovine Bone

• Sinónimos: Osteocalcin, Bovine Bone, Bone-Gla-Protein, BGP
• Fórmula empírica (notación de Hill): C₂₆₃H₃₇₂N₆₆O₈₂S₂
• Peso molecular: 5834.29
• Código UNSPSC: 12352202

Propiedades

• Análisis: ≥98% (SDS-PAGE)
• Nivel de calidad: 100
• formulario: liquid
• fabricante / nombre comercial: Calbiochem^®
• condiciones de almacenamiento: OK to freeze; avoid repeated freeze/thaw cycles
• pI: 4.0 - 4.5
• Condiciones de envío: wet ice
• temp. de almacenamiento: −20°C

Descripción

Descripción general

Single chain vitamin K-dependent protein produced by osteoblasts. Osteocalcin purified from bovine bone. Binds to phospholipid vesicles in the presence of Ca²⁺ (K_d = 6 µM). Also binds hydroxylapatite. A good marker of bone turnover. May play a role in bone mineralization, bone resorption, and bone formation.

Single chain vitamin K-dependent protein produced by osteoblasts. Osteocalcin purified from bovine bone. Binds to phospholipid vesicles in the presence of Ca²⁺ (K_d = 6 µM). Also binds hydroxylapatite. Possible role in bone mineralization, bone resorption, and bone formation. Note: Gla corresponds to γ-carboxyglutamic acid.

Advertencia

Toxicity: Standard Handling (A)

Secuencia

H-Tyr-Leu-Asp-His-Trp-Leu-Gly-Ala-Pro-Ala-Pro-Tyr-Pro-Asp-Pro-Leu-Gla-Pro-Lys-Arg-Gla-Val-Cys²³-Gla-Leu-Asn-Pro-Asp-Cys²⁹-Asp-Glu-Leu-Ala-Asp-His-Ile-Gly-Phe-Gln-Glu-Ala-Tyr-Arg-Arg-Phe-Tyr-Gly-Pro-Val-OH (disulfide bond: 23 → 29)

Forma física

In 75 mM NaCl, 10 mM sodium phosphate buffer, pH 7.4.

Reconstitución

Following initial thaw, aliquot and freeze (-20°C).

Otras notas

Carter, S.D., et al. 1996. J. Anim. Sci. 74, 2719.
Watson, K.E., et al. 1994. J. Clin. Invest.93, 2106.
Tracy, R.P., et al. 1990. J. Bone Min. Res.5, 451.
Gendreau, M.A., et al. 1989. J. Biol. Chem.264, 6972.

Información legal

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Información de seguridad

• Código de clase de almacenamiento: 10 - Combustible liquids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable