444810
ProteoExtract® Native Membrane Protein Extraction Kit
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About This Item
Código UNSPSC:
41106500
Productos recomendados
uso
sufficient for 20 extractions
fabricante / nombre comercial
Calbiochem®
condiciones de almacenamiento
OK to freeze
técnicas
protein extraction: suitable
entrada
sample type: mammalian tissue
Condiciones de envío
ambient
temp. de almacenamiento
2-8°C
Descripción general
A convenient kit for the isolation of native integral membrane and membrane-associated proteins, without the need for ultracentrifugation. Extraction is based on association of proteins with cellular membranes rather than on their hydrophobicity. Resulting samples are suitable for use in functional assays, 2-D gel electrophoresis, and other applications.
Membrane proteins represent only one-third of the proteins encoded by the human genome, but they represent more than two-thirds of the known protein targets for drugs. Therefore, approaches to prepare and characterize membrane proteins are of significant interest for drug discovery. However, due to their hydrophobic nature, membrane proteins are more difficult to analyze than soluble proteins. In addition to the intrinsic difficulty of solubilization, membrane proteins are challenging because of their general low abundance. Thus, the challenges for sample preparation of membrane proteins are effective solubilization and selective enrichment, ideally keeping proteins in a non-denatured state.
Note: 1 kit is sufficient for up to 20 sample extractions.
Componentes
Wash Buffer, Extraction Buffer 1, Extraction Buffer 2, Protease Inhibitor Cocktail, and a user protocol.
Advertencia
Toxicity: Multiple Toxicity Values, refer to MSDS (O)
Principio
The ProteoExtract® Native Membrane Protein Extraction Kit is designed for the isolation of native membrane proteins from a broad range of mammalian samples, including:
• Adherent tissue culture cells
• Suspension grown tissue culture cells
• Frozen cell pellets
• Tissues
• Adherent tissue culture cells
• Suspension grown tissue culture cells
• Frozen cell pellets
• Tissues
Nota de preparación
The amount of buffer required for each extraction is dependent upon the amount of starting cell material.
Almacenamiento y estabilidad
• Wash Buffer
Store at 4°C.
• Extraction Buffers I & II
The Extraction Buffers I and II can be stored at 4°C for up to 6 month.
For prolonged storage, freeze the buffers in convenient aliquots at -20°C. Before extraction, buffers must be thawed at room temperature (RT). After thawing, mix components by gently shaking or vortexing. Avoid repeated freezing and thawing!
• Protease Inhibitor Cocktail
The Protease Inhibitor Cocktail is supplied in DMSO and can be stored at 4°C up to 6 months. For prolonged storage, freeze the cocktail in convenient aliquots at -20°C. During the sample preparation procedure it must be kept at RT to prevent freezing of DMSO.
Store at 4°C.
• Extraction Buffers I & II
The Extraction Buffers I and II can be stored at 4°C for up to 6 month.
For prolonged storage, freeze the buffers in convenient aliquots at -20°C. Before extraction, buffers must be thawed at room temperature (RT). After thawing, mix components by gently shaking or vortexing. Avoid repeated freezing and thawing!
• Protease Inhibitor Cocktail
The Protease Inhibitor Cocktail is supplied in DMSO and can be stored at 4°C up to 6 months. For prolonged storage, freeze the cocktail in convenient aliquots at -20°C. During the sample preparation procedure it must be kept at RT to prevent freezing of DMSO.
Información legal
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
PROTEOEXTRACT is a registered trademark of Merck KGaA, Darmstadt, Germany
Palabra de señalización
Warning
Frases de peligro
Consejos de prudencia
Clasificaciones de peligro
Aquatic Chronic 3 - Eye Irrit. 2 - Skin Irrit. 2
Código de clase de almacenamiento
10 - Combustible liquids
Clase de riesgo para el agua (WGK)
WGK 2
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
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