444248

MMP-13, Proenzyme, His•Tag^®, Human, Recombinant, S. frugiperda

• Sinónimos: Collagenase-3
• Código UNSPSC: 12352202
• NACRES: NA.77

Propiedades

• Análisis: ≥90% (SDS-PAGE)
• Nivel de calidad: 100
• formulario: liquid
• actividad específica: ≥50 mU/mg protein
• fabricante / nombre comercial: Calbiochem^®
• condiciones de almacenamiento: OK to freeze; avoid repeated freeze/thaw cycles
• Condiciones de envío: wet ice
• temp. de almacenamiento: −70°C

Descripción

Descripción general

Full-length recombinant, human MMP-13 fused to a His•Tag sequence and expressed in S. frugiperda insect cells. This 452 amino acid proenzyme contains an N-terminal propeptide which confers latency to the proenzyme, a Ca²⁺- and Zn²⁺- binding catalytic domain, a hinge region, and a C-terminal hemopexin domain. Hydrolyzes collagen type II 5-6 times faster than collagens type I and III. Exhibits high activity towards gelatin and degrades α1-antichymotrypsin and plasminogen activator inhibitor-2.

Full-length recombinant, human MMP-13 fused to a His•Tag sequence and expressed in S. frugiperda insect cells. This 452 amino acid proenzyme contains the N-terminal propeptide, the Ca²⁺- and Zn²⁺-binding catalytic domain, the hinge region, and the C-terminal hemopexin domain. Hydrolyzes collagen type II 5 to 6 times faster than collagens Type I and III. Exhibits high activity towards gelatin and degrades α₁-antichymotrypsin and plasminogen activator inhibitor-2 (PAI-2).

Note: 1 mU = 1 milliunit.

Advertencia

Toxicity: Standard Handling (A)

Definición de unidad

One unit is defined as the amount of APMA-activated enzyme that will hydrolyze 1.0 µmol MCA-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH₂ (Cat. No. 03-32-5032) per min at 37°C, pH 7.5.

Forma física

In 150 mM NaCl, 50 mM Tris-HCl, 5 mM CaCl₂, pH 7.5.

Reconstitución

Following initial thaw, aliquot and freeze (-70°C).

Otras notas

Herget, J., et al. 2003. Am. J. Physiol.285, 4199.
Nagase, H. 1997. Biol. Chem.378, 151.
Knäuper, V., et al. 1996. J. Biol. Chem. 271, 1544.
Knauper, V., et al. 1996. J. Biol. Chem.271, 17124.
Pei, D., and Weiss, S.J. 1996. J. Biol. Chem.271, 9135.
Knight, C.G., et al. 1992. FEBS Lett.296, 263.

Información legal

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

HIS TAG is a registered trademark of Merck KGaA, Darmstadt, Germany

Información de seguridad

• Código de clase de almacenamiento: 10 - Combustible liquids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable