444234

MMP-9-Lipocalin-TIMP-1 Complex, Human Neutrophil

• Sinónimos: Matrix Metalloproteinase 9-Lipocalin-TIMP-1 Complex
• Código UNSPSC: 12352202
• NACRES: NA.77

Propiedades

• Análisis: ≥90% (SDS-PAGE)
• Nivel de calidad: 100
• formulario: liquid
• actividad específica: ≥600 mU/mg protein
• fabricante / nombre comercial: Calbiochem^®
• condiciones de almacenamiento: OK to freeze; avoid repeated freeze/thaw cycles
• actividad extraña: other MMP activity, none detected
• Condiciones de envío: wet ice
• temp. de almacenamiento: −70°C

Descripción

Descripción general

Native MMP-9-Lipocalin-TIMP-1 complex from stimulated human neutrophils. The ternary MMP-9/lipocalin/TIMP-1 complex behaves like the Pro-MMP-9/TIMP-1 complex, acting as an inhibitor of active MMPs. Following activation, the complex acts as a gelatinase, with reduced activity.

Native MMP-9-Lipocalin-TIMP-1 complex from stimulated human neutrophils. The ternary MMP-9/lipocalin/TIMP-1 complex behaves like the progelatinase B/TIMP-1 complex in acting as an inhibitor of active MMPs. Following activation, the complex acts as a gelatinase with reduced activity.

Note: 1 mU = 1 milliunit.

Advertencia

Toxicity: Standard Handling (A)

Definición de unidad

One unit is defined as the amount of enzyme that will hydrolyze 1.0 µmol 2,4-DNP-Pro-Gln-Gly-Ile-Ala-Gly-Gln-D-Arg-OH per min at 37°C, pH 7.0.

Forma física

In 200 mM NaCl, 50 mM Tris-HCl, 5 mM CaCl₂, 1 µM ZnCl₂, 0.05% BRIJ^® 35 Detergent, 0.05% NaN₃, pH 7.0.

Nota de preparación

Prepared from stimulated neutrophils that have been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.

Reconstitución

Following initial thaw, aliquot and freeze (-70°C).

Otras notas

Kolkenbrock, H., et al. 1996. Biol. Chem. 377, 529.

Información legal

Brij is a registered trademark of Croda International PLC

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Información de seguridad

• Código de clase de almacenamiento: 10 - Combustible liquids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable