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Key Documents

420220-M

Millipore

Thiazovivin

≥98% (HPLC), powder, Rho kinase inhibitor, Calbiochem

Sinónimos:

iPSC Induction Enhancer, Thiazovivin, N-Benzyl-2-(pyrimidin-4-ylamino)thiazole-4-carboxamide, Induced Pluripotent Stem Cells Induction Enhancer, Rho Kinase Inhibitor XII

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About This Item

Fórmula empírica (notación de Hill):
C15H13N5OS
Peso molecular:
311.36
Código UNSPSC:
12352200

product name

iPSC Induction Enhancer, Thiazovivin, A cell-permeable aminothiazolo-carboxamide compound that is reported to augment SB431542 and PD0325901.

Nivel de calidad

Análisis

≥98% (HPLC)

formulario

powder

fabricante / nombre comercial

Calbiochem®

condiciones de almacenamiento

OK to freeze
protect from light

color

tan

solubilidad

DMSO: 100 mg/mL

temp. de almacenamiento

2-8°C

Descripción general

A cell-permeable aminothiazolo-carboxamide compound that is ~5-fold more potent than Y-27632 (Cat. Nos. 688000 & 688001) in inhibiting ROCK enzymatic activity (71% inhibition at 2 µM). Single-cell dissociation of cultured hESCs is demonstrated to hyperactivate Rho-ROCK signaling and result in cell death, while suppression of such hyperactivation by Tzv (2 µM) or Y-27632 (2 µM) stabilize newly synthesized cell surface E-cadherin, which in turn promotes cell-cell adhesion and prevents trypsinization-induced apoptosis (12% vs 81% TUNEL-positive cells after trypsinization in matrigel cultures with 2 µM Tzv or DMSO, respectively). Unlike, Ptn (Cat. No. 544049), Tzv protects dissociated individual hESCs even in the absence of ECM (24% vs 90% TUNEL-positive cells after trypsinization in non-coating plates with 2 µM Tzv or Ptn, respectively). Simultaneous blockage of TGFβ and MEK-ERK pathways by 2 µM SB431542 (Cat. No. 616461)and 0.5 µM PD0325901 (Cat. No. 444966) is reported to result in >50-fold improvement of human iPSCs (induced Pluripotent Stem Cells) generation from fibroblast cultures virally transfected with 4-TF (Oct44, Klf4, Sox2, and c-Myc), while the inclusion of 0.5 µM Tzv on top of SB431542 and PD0325901 is demonstrated to further improve the efficiency to an overall improvement of >200-fold from 4-TF-induction alone.
A cell-permeable aminothiazolo-carboxamide compound that is ~5-fold more potent than Y-27632 (Cat. Nos. 688000 & 688001) as a ROCK inhibitor (71% inhibition at 2 µM). Effectively prevents trypsinization-induced apoptosis in hESC cultures by stabilizing newly synthesized cell surface E-cadherin and cell-cell adhesion. Unlike, Ptn (Cat. No. 544049), Tzv protects dissociated hESCs even in the absence of ECM. Greatly augments SB431542 (Cat. No. 616461)and PD0325901 (Cat. No. 444966) in improving iPSCs generation efficiency from 4-TF- (Oct44, Klf4, Sox2, and c-Myc) transfected fibroblast cultures.

Advertencia

Toxicity: Standard Handling (A)

Reconstitución

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Otras notas

Xu, Y., et al. 2010. Proc. Natl. Acad. Sci. USA107, 8129
Lin, T., et al. 2009. Nat. Methods6, 805.

Información legal

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Código de clase de almacenamiento

11 - Combustible Solids

Clase de riesgo para el agua (WGK)

WGK 3

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable


Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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