411550

Mouse Anti-Human IgG, Fc Fragment Specific (HP6043) Peroxidase Conjugate

liquid, clone HP6043, Calbiochem^®

• Código UNSPSC: 12352203
• NACRES: NA.46

Propiedades

• origen biológico: mouse
• Nivel de calidad: 200
• forma del anticuerpo: purified antibody
• tipo de anticuerpo: secondary antibodies
• clon: HP6043, monoclonal
• formulario: liquid
• no contiene: preservative
• fabricante / nombre comercial: Calbiochem^®
• condiciones de almacenamiento: OK to freeze; avoid repeated freeze/thaw cycles
• isotipo: IgG
• Condiciones de envío: wet ice
• temp. de almacenamiento: −20°C

Descripción

Descripción general

Purified mouse monoclonal antibody conjugated to horseradish peroxidase. Recognizes human IgG Fc fragment.

This Mouse Anti-Human IgG, Fc Fragment Specific (HP6043) Peroxidase Conjugate is validated for use in Enzyme Immunoassay for the detection of Human IgG, Fc Fragment Specific.

Aplicación

Enzyme Immunoassay (1:1000, see application references)

Envase

Please refer to vial label for lot-specific concentration.

Advertencia

Toxicity: Standard Handling (A)

Forma física

In PBS, 10 mg/ml BSA.

Otras notas

Antibody should be titrated for optimal results in individual systems.



Cross-reactivity by ELISA against human myeloma proteins:

Human IgG₁: 100%

Human IgG₂: 100%

Human IgG₃: 100%

Human IgG₄: 100%

Human IgM: <0.2%

Human IgA: <0.1%

Human IgE: <0.1%



Cross-reactivity by ELISA against chimeric antibodies:

Human IgG₁: 100%

Human IgG₂: 100%

Human IgG₃: 100%

Human IgG₄: 100%

Human IgM: <0.01%

Human IgA: <0.01%

Human IgE: <0.01%



There is no detectable binding to bovine, goat, horse sheep IgG, so these species sera can be used for assay blocking buffers. Modification of Nakane and Kawaoi method used for enzyme coupling.

Hamilton, R.G. 1993. Clin. Chem.39, 1988.
Hamilton, R.G. and Morrison, S.M. 1993. J. Immunolo. Meth.158, 107.
Hamilton, R.G. 1990. Ann. Biol. Clin.48, 473.
Hamilton, R.G., et al. 1988. J. Immunoassay.9, 275.
Hamilton, R.G., et al. 1987. Hybridoma6, 204.
Nakane, P.K. and Kawaoi A., 1974. J. Histochem Cytochem.22, 1084.

Información legal

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 2
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable