374817

Hemocyanin, Keyhole Limpet, Megathura crenulata, Glycerol

Hemocyanin, Keyhole Limpet, Megathura crenulata, Glycerol, is a large, multi-subunit, oxygen-carrying, metalloprotein that is used as a carrier protein in the production of antibodies.

• Sinónimos: Hemocyanin, Keyhole Limpet, Megathura crenulata, Glycerol, KLH
• Número de CAS: 9013-72-3
• Número MDL: MFCD00131280
• Código UNSPSC: 12352202
• NACRES: NA.25

Propiedades

• Nivel de calidad: 100
• Análisis: >90% (size exclusion chromatography)
• formulario: liquid
• fabricante / nombre comercial: Calbiochem^®
• condiciones de almacenamiento: OK to freeze
• color: clear blue-gray
• Condiciones de envío: ambient
• temp. de almacenamiento: 2-8°C

Descripción

Descripción general

Carrier protein that has many available primary amines to facilitate protein conjugation. Used to immunize animals to elicit antibodies to small molecules (haptens) by covalent conjugation. Protein: ≥10 mg/ml. Sold on the basis of protein content.

Envase

Please refer to vial label for lot-specific concentration.

Advertencia

Toxicity: Standard Handling (A)

Forma física

In 10 mM MgSO₄, 5 mM BES, 50% glycerol, 0.9% sodium chloride, pH 6.5.

Nota de preparación

Further dilute with PBS or physiological saline. Material can be dialyzed to remove the glycerol or used as is for conjugation with glutaraldehyde, carbodiimide, or mixed anhydrides.KLH is an extremely large protein complex that has a tendency to aggregate and precipitate from solutions. Use a buffer when diluting KLH and maintain the magnesium concentration at ≥10 mM. Store KLH solutions at 4°C. DO NOT freeze, vortex or agitate to dissolve KLH. Freezing or agitating KLH will cause protein aggregation and precipitation.

Otras notas

Hayashi, K., et al. 1992. Virchows Archiv. B Cell Pathol.63, 37.
Shuler, K.R., et al. 1992. J. Immunol. Methods156, 137.

Información legal

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Información de seguridad

• Código de clase de almacenamiento: 10 - Combustible liquids
• Clase de riesgo para el agua (WGK): WGK 2