362185

N-Glycosidase F, Elizabethkingia meningosepticum

• Sinónimos: N-Glycosidase F, Elizabethkingia meningosepticum, Glycopeptidase F, PNGase F
• Número de CAS: 83534-39-8
• Comisión internacional de enzimas: 3.5.1.52 (BRENDA, IUBMB)
• Número MDL: MFCD00131222
• Código UNSPSC: 12352202
• NACRES: NA.54

Propiedades

• origen biológico: bacterial (Elizabethkingia meningosepticum)
• Nivel de calidad: 100
• conjugado: (N-linked)
• formulario: liquid
• actividad específica: ≥20,000 units/mg protein; ≥4500 units/mL
• fabricante / nombre comercial: Calbiochem^®
• condiciones de almacenamiento: do not freeze
• actividad extraña: Proteases, none detected
• Condiciones de envío: wet ice
• temp. de almacenamiento: 2-8°C

Descripción

Descripción general

Native N-Glycosidase F from Elizabethkingia meningosepticum. Catalyzes the hydrolysis of asparagine-linked high mannose, as well as hybrid and complex oligosaccharides from glycoproteins. This enzyme will not cleave oligosaccharides containing α1,3-linked core fucose commonly found on plant glycoproteins. N-Glycosidase F will also fail to cleave if the asparagine to which the oligosaccharide is attached is not peptide-bonded to at least one amino acid residue at both the amino and carboxy termini.

Native N-Glycosidase F from Elizabethkingia meningosepticum. Catalyzes the hydrolysis of asparagine-linked high mannose, as well as hybrid and complex oligosaccharides from glycoproteins. This enzyme will not cleave oligosaccharides containing α1-3-linked core fucose commonly found on plant glycoproteins. N-Glycosidase F will also fail to cleave if the asparagine to which the oligosaccharide is attached is not peptide-bonded to at least one amino acid residue at both the amino and carboxy termini.

Advertencia

Toxicity: Standard Handling (A)

Definición de unidad

One unit is defined as the amount of enzyme that will catalyze the release of N-linked oligosaccharides from 1.0 nmol denatured ribonuclease B per min at 37°C, pH 7.5. Cleavage is monitored by SDS-PAGE. One unit of N-Glycosidase F is equal to 1 IUB milliunit.

Forma física

In 50 mM NaCl, 20 mM Tris-HCl, 1 mM EDTA, pH 7.5.

Otras notas

Means, R.E. and Desrosiers, R.C. 2000. J. Virol.74, 11181.
Fan, J.Q., and Lee, Y.C. 1997. J. Biol. Chem. 272, 27058.
Tarentino, A.L., and Plummer, T.H. 1994. Methods Enzymol. 230, 44.
Trimble, R.B., and Tarentino, A.L. 1991. J. Biol. Chem. 266, 1646.

Información legal

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 2
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable