345852

Geranylgeranyltransferase I, GST-Fusion, His•Tag^®, Rat, Recombinant, E. coli

Geranylgeranyltransferase I, GST-Fusion, His•Tag, Rat, Recombinant, E. coli, contains a 74 kDa α- & 43 kDa β-subunit. The α-subunit is expressed as a GST-fusion construct with an N-terminal His•Tag.

• Sinónimos: GGTase I
• Código UNSPSC: 12352202
• NACRES: NA.54

Propiedades

• recombinante: expressed in E. coli
• Nivel de calidad: 100
• Ensayo: ≥90% (SDS-PAGE)
• Formulario: liquid
• fabricante / nombre comercial: Calbiochem^®
• condiciones de almacenamiento: OK to freeze; avoid repeated freeze/thaw cycles
• Condiciones de envío: wet ice
• temp. de almacenamiento: −70°C

Descripción

Descripción general

Recombinant, rat GGTase I composed of an α- and a β-subunit (74 and 43 kDa, respectively). The α-subunit is expressed as a GST-fusion construct with an N-terminal His•Tag sequence. Both the GST moiety and the His•Tag sequence can be utilized to facilitate enzyme capturing in prenylation and binding studies or, when desired, can also be cleaved by TEV protease. Requires Zn²⁺ for optimal activity. The enzyme is purified with Zn²⁺ tightly bound, so addition of Zn²⁺ to the reaction mix is not necessary unless further manipulation of the enzyme results in release of the ions.

Recombinant, rat GGTase I composed of an α- and a β-subunit (74 and 43 kDa, respectively). The α-subunit is expressed as a GST-fusion construct with an N-terminal His•Tag sequence. Both the GST moiety and the His•Tag sequence can be utilized to facilitate enzyme capturing in prenylation and binding studies or, when desired, can also be cleaved off by TEV protease. Requires Zn²⁺ for optimal activity.

Acciones bioquímicas o fisiológicas

1 pmol enzyme will transfer ≥3 pmol geranylgeranyl to RhoA in 10 min at 37°C, pH 7.2.

Advertencia

Toxicity: Standard Handling (A)

Forma física

In 40 mM NaCl, 25 mM HEPES, 5 mM DTT, pH 7.2.

Reconstitución

Following initial thaw, aliquot and freeze (-70°C).

Otras notas

Kalinin, A., et al. 2001. Protein Expr. Purif.22, 84.
Zhang, F.L., et al. 1994. J. Biol. Chem.269, 23465.
Moomaw, J.F., and Casey, P.J., 1992. J. Biol. Chem.267, 17438.

Información legal

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

HIS TAG is a registered trademark of Merck KGaA, Darmstadt, Germany

Información de seguridad

• Código de clase de almacenamiento: 10 - Combustible liquids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable