344826

α1,6-Fucosidase, Chryseobacterium meningosepticum, Recombinant, E. coli

This enzyme from Chryseobacterium (formerly Flavobacterium) meningosepticum catalyzes the hydrolysis of branched non-reducing terminal α1,6-linked fucose residues from the N-acetylglucosamine core of N-Linked oligosaccharides.

• Sinónimos: α1,6-Fucosidase, Chryseobacterium meningosepticum, Recombinant, E. coli, α-L-Fucoside Fucohydrolase
• Número de CAS: 9037-65-4
• Comisión internacional de enzimas: 3.2.1.51 (BRENDA, IUBMB)
• Código UNSPSC: 12352202
• NACRES: NA.54

Propiedades

• recombinante: expressed in E. coli
• Nivel de calidad: 100
• Formulario: liquid
• actividad específica: ≥1.0 units/mL; ≥1.5 units/mg protein
• fabricante / nombre comercial: Calbiochem^®
• condiciones de almacenamiento: do not freeze
• actividad extraña: Proteases, none detected
• Condiciones de envío: wet ice
• temp. de almacenamiento: 2-8°C

Descripción

Descripción general

Note: 1 mU = 1 milliunit.

Recombinant, Chryseobacterium meningosepticum α1,6-fucosidase expressed in E. coli. This enzyme from Chryseobacterium (formerly Flavobacterium) meningosepticum catalyzes the hydrolysis of branched non-reducing terminal α1,6-linked fucose residues from the N-acetylglucosamine core of N-Linked oligosaccharides. The reducing terminus of the oligosaccharide must be covalently bonded to a reporter molecule, such as aminonaphthalene disulfonic acid (ANDSA), aminonaphthalene trisulfonate (ANTS), and 2-aminoanthanilic acid (2-AA). The one exception is that a terminal unbranched α1,3-fucose is cleaved in the absence of any reporter molecule. Does not cleave fucose from glycoproteins.

Recombinant, Chryseobacterium meningosepticum α1,6-fucosidase expressed in E. coli. This enzyme from Chryseobacterium (formerly Flavobacterium meningosepticum) catalyses the hydrolysis of branched non-reducing terminal α1,6-linked fucose residues from the N-acetylglucosamine core of N-linked oligosaccharides. The reducing terminus of the oligosaccharide must be covalently bonded to a reporter molecule, such as aminonaphthalene disulfonic acid (ANDSA), aminonaphthalene trisulfonate (ANTS), and 2-aminoanthanilic acid (2-AA). The one exception is that a terminal unbranched α1,3-fucose is cleaved in the absence of any reporter molecule. Does not cleave fucose from glycoproteins.

Advertencia

Toxicity: Standard Handling (A)

Definición de unidad

One unit is defined as the amount of enzyme that will release 1.0 µmol methylumbelliferone from 4-methylumbelliferyl-α-L-fucoside per min at 37°C, pH 5.0.

Forma física

In 25 mM NaCl, 20 mM Tris-HCl, pH 7.5.

Otras notas

Zheng, Q., et al. 2002. J. Biol. Chem.277, 39823.

Información legal

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 2
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable