325885

c-MET, GST-Fusion, Human, Recombinant, S. frugiperda

c-MET, GST-Fusion, Human, Recombinant (aa 956-1390) fused at the N-terminus to a GST-His6-thrombin cleavage site and expressed in S. frugiperda. c-Met is a receptor tyrosine kinase activated by HGF.

• Sinónimos: Hepatocyte growth factor receptor tyrosine kinase
• Código UNSPSC: 12352202
• NACRES: NA.77

Propiedades

• recombinante: expressed in baculovirus infected Sf21 cells
• Nivel de calidad: 100
• Formulario: liquid
• actividad específica: ≥30 units/mg protein
• fabricante / nombre comercial: Calbiochem^®
• condiciones de almacenamiento: OK to freeze; avoid repeated freeze/thaw cycles
• Condiciones de envío: wet ice
• temp. de almacenamiento: −70°C

Descripción

Descripción general

Recombinant, human MET (amino acids 956-1390) (Genebank entry NM_000245) fused at the N-terminus to a GST-His₆-thrombin cleavage site sequence and expressed in S. frugiperda insect cells using a baculovirus expression system. c-Met is a receptor tyrosine kinase activated by hepatocyte growth factor (HGF)/scatter factor (SF). MET is a plasminogen-like protein thought to be a humoral mediator of liver regeneration.

Recombinant, human c-MET (amino acids 956-1390) (Genebank entry NM_000245) fused at the N-terminus to a GST-His₆-thrombin cleavage site sequence and expressed in S. frugiperda insect cells using a baculovirus expression system. c-Met is a receptor tyrosine kinase activated by hepatocyte growth factor (HGF)/scatter factor (SF). c-MET is a plasminogen-like protein thought to be a humoral mediator of liver regeneration.

Envase

Please refer to vial label for lot-specific concentration.

Advertencia

Toxicity: Standard Handling (A)

Definición de unidad

One unit is defined as the amount of enzyme required to transfer 1 nmol phosphate to Poly(Ala, Glu, Lys, Tyr)_6:2:5:1 per min at 30°C, using variable concentrations of ATP (0.1-1.48 µM).

Forma física

In 100 mM NaCl, 50 mM Tris-HCl, 5 mM DTT, 4 mM reduced glutathione, 20% glycerol, pH 8.0.

Reconstitución

Following initial thaw, aliquot and freeze (-70°C).

Otras notas

Palka, H.L., et al. 2003. J. Biol. Chem.278, 5278.
Vadnais, J., et al. 2002. J. Biol. Chem.277, 48342.
Crostella, L., et al. 2001. Oncogene20, 3735.
Guiton, M., et al. 1994. J. Biol. Chem269, 30370.

Información legal

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Información de seguridad

• Código de clase de almacenamiento: 10 - Combustible liquids
• Clase de riesgo para el agua (WGK): WGK 1