324393
Anti-Dopamine D₂ Receptor Rabbit pAb
lyophilized, Calbiochem®
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About This Item
Código UNSPSC:
12352203
NACRES:
NA.41
origen biológico
rabbit
Nivel de calidad
forma del anticuerpo
serum
tipo de anticuerpo
primary antibodies
clon
polyclonal
formulario
lyophilized
no contiene
preservative
reactividad de especies
rat, human
fabricante / nombre comercial
Calbiochem®
condiciones de almacenamiento
OK to freeze
isotipo
IgG
Condiciones de envío
ambient
temp. de almacenamiento
2-8°C
modificación del objetivo postraduccional
unmodified
Información sobre el gen
human ... DRD2(1813)
rat ... Drd2(24318)
Descripción general
Anti-Dopamine D₂ Receptor, rabbit polyclonal, specifically recognizes the dopamine D2 receptor in rat brain. Has minimal reactivity with the D2S receptor. It is validated for use in ELISA, WB, & IHC.
Rabbit polyclonal antibody supplied as lyophilized, undiluted serum. Recognizes the ~48-51 kDa dopamine D2 receptor protein.
Recognizes the dopamine D2 receptor in rat brain. Does not cross-react with other dopamine receptors and exhibits minimal cross-reactivity with the D2S short receptor.
Inmunógeno
Human
a synthetic peptide (CAARRAQELEME) corresponding to amino acids 272-282 of human D₂ dopamine receptor
Aplicación
ELISA (see comments)
Immunoblotting (1:800)
Frozen Sections (1:5000, see application references)
Immunoblotting (1:800)
Frozen Sections (1:5000, see application references)
Advertencia
Toxicity: Standard Handling (A)
Forma física
Undiluted serum.
Reconstitución
Reconstitute lyophilized antibody with 100 µl PBS containing 10 mg/ml BSA. For further dilution of the antibody use a similar buffer formulation. Following reconstitution, refrigerate (4°C).
Nota de análisis
Positive Control
Rat brain
Rat brain
Otras notas
Mignini, F., et al. 2000. J. Autonom. Pharmacol.20, 325.
This antibody is highly specific for the immunizing peptide sequence and is suitable for detection of both the D2S and D2L dopamine receptors in human and rat samples. This antibody has also been reported to work for ELISA. For immunoblotting, a 1:800 dilution of the reconstituted antiserum detects two bands at ~48 and ~51 kDa in an analysis using whole rat brain homogenates. For blocking immunoblot membranes, use ONLY 4% goat serum or evaporated goat′s milk. For frozen sections, a 1:5000 dilution of the reconstituted antiserum demonstrates specific staining of rat brain sections including the plasma membrane in the cortex, caudate putamen, globus pallidus, thalamic nucleus, supra optic nucleus, and the dentate gyrus. Variables associated with assay conditions will dictate the proper working dilution.
Información legal
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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Código de clase de almacenamiento
11 - Combustible Solids
Clase de riesgo para el agua (WGK)
WGK 2
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
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