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Key Documents

234401

Sigma-Aldrich

Complement C1q-Depleted Serum, Human

Sinónimos:

C1q-Depleted Serum, Human Complement Serum

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About This Item

Código UNSPSC:
12352202
NACRES:
NA.25

formulario

solution

Nivel de calidad

no contiene

preservative

fabricante / nombre comercial

Calbiochem®

condiciones de almacenamiento

OK to freeze
avoid repeated freeze/thaw cycles

impurezas

C1q, none detected (Ouchterlony using various dilutions of goat anti-human C1q antiserum.)

color

clear amber

Condiciones de envío

dry ice

temp. de almacenamiento

−70°C

Descripción general

Human serum that has been depleted of C1q complement component.
Human serum that has been depleted of C1q complement component. C1q-depleted human serum, sterile filtered through a 0.22 µm filter. This product has the following specifications:

Envase

Please refer to vial label for lot-specific concentration.

Advertencia

Toxicity: Standard Handling (A)

Definición de unidad

Classical Pathway Activity: One C1qH50 unit measured by classical pathway activation is defined as the amount of C1q required to yield 50% lysis of 3 x 10⁷ EA when incubated in the presence of the recommended volume of C1q-Dpl for 30 min at 37°C in a total reaction volume of 500 µl GVB++.CH50 Functional Activity upon Reconstitution: One CH50 unit is defined as the input of C1q-Dpl, reconstituted with C1q, or NHS Complement Standard yielding 50% lysis of 1 x 10⁸ EA when incubated for 60 min at 37°C in a total reaction volume of 1.5 ml GVB++.Alternative Pathway Activity: One unit of whole alternative pathway activity (APH50) is defined as the input of C1q-Dpl or NHS Complement Standard yielding 50% lysis of 1.5 x 10⁷ rabbit erythrocytes when incubated for 30 min at 37°C in a total reaction volume of 75 ml GVB° containing a final Mg-EGTA concentration of 13.3 mM.

Forma física

In PBS, ≤0.1% sodium azide, pH 7.4.

Nota de preparación

Prepared from serum that has been shown by certified tests to be negative for HBsAg and for antibodies to HCV, HIV-I, and HIV-II.

Reconstitución

Following initial thaw, aliquot and freeze (-70°C).

Otras notas

No C1q detected by Ouchterlony using various dilutions of goat anti-human C1q antiserum. The following information will vary from lot to lot and can be found on the certificate of analysis:



CLASSICAL PATHWAY ACTIVITY: One C1qH50 unit measured by classical pathway activation is defined as the amount of C1q required to yield 50% lysis of 3 x 107 EA when incubated in the presence of the recommended volume of C1q-Dpl for 30 minutes at 37°C in a total reaction volume of 500 μl GVB++.

• Recommended volume of C1q-Dpl serum per assay

• C1qH50 units/mg purified C1q at the recommended input of C1q-Dpl

• Input of purified C1q to yield 1 C1qH50:

• C1qH50 units/ml NHS Complement standard at the recommended input of C1q-Dpl

• Input of C1q in NHS Complement standard to yield 1 C1qH50

• Ratio

• Background A412 EA blank reading at the recommended input of C1q-Dpl



CH50 FUNCTIONAL ACTIVITY UPON RECONSTITUTION: One CH50 unit is defined as the input of C1q-Dpl, reconstituted with C1q, or NHS Complement Standard yielding 50% lysis of 1 x 108 EA when incubated for 60 minutes at 37°C in a total reaction volume of 1.5 ml GVB++.

• CH50/ml NHS Standard

• CH50/ml C1q-Dpl reconstituted with 100 μg C1q/ml:

• Ratio



ALTERNATIVE PATHWAY ACTIVITY: One unit of whole alternative pathway activity (APH50) is defined as the input of C1q-Dpl or NHS Complement Standard yielding 50% lysis of 1.5 x 107 rabbit erythrocytes when incubated for 30 minutes at 37°C in a total reaction volume of 75 ml GVB° containing a final Mg-EGTA concentration of 13.3 mM.

• APH50/ml NHS Standard

• Input of NHS Standard to yield 1 APH50

• APH50/ml C1q-Dpl

• Input of C1q-Dpl to yield 1 APH50

• AP Ratio

Información legal

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Código de clase de almacenamiento

12 - Non Combustible Liquids

Clase de riesgo para el agua (WGK)

nwg

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable


Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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