218807

Caspase-9, Human, Recombinant, E. coli

Caspase-9, Human, Recombinant is expressed in E. coli as a single polypeptide. Fused at the N- and C- termini to a His•Tag sequence. Undergoes spontaneous autoprocessing to yield the active enzyme.

• Sinónimos: ICE-LAP6, Mch6
• Código UNSPSC: 12352202

Propiedades

• recombinante: expressed in E. coli
• Nivel de calidad: 100
• Análisis: ≥70% (SDS-PAGE)
• formulario: lyophilized
• actividad específica: ≥400 units/mg protein
• fabricante / nombre comercial: Calbiochem^®
• condiciones de almacenamiento: OK to freeze
• solubilidad: PBS with 15% glycerol: 1 units/μL
• Condiciones de envío: wet ice
• temp. de almacenamiento: −70°C

Descripción

Descripción general

Recombinant, human caspase-9 fused at the N- and C- termini to a His•Tag sequence and expressed in E. coli as a single polypeptide. Subsequently undergoes spontaneous autoprocessing to yield the active enzyme. MW: 11000 and 20000.

Advertencia

Toxicity: Standard Handling (A)

Definición de unidad

One unit is defined as the amount of enzyme that will cleave 1.0 nmol substrate Ac-LEHD-pNA (Cat. No. 218805) per h at 37°C, pH 7.2.

Forma física

Lyophilized from 130 mM NaCl, 20 mM Tris, 4 mM (NH₄)₂SO₄

Reconstitución

Following reconstitution, aliquot and freeze (-70°C). Avoid freeze/thaw cycles of solutions. Stock solutions are stable for up to 4 months at -20°C.

Otras notas

Qin, H., et al. 1999. Nature399, 549.
Li, P., et al. 1997. Cell91, 479.
Salvesen, G.S., et al. 1997. Cell91, 443.

Información legal

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Información de seguridad

• Código de clase de almacenamiento: 11 - Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 1