208731

Calpain Substrate II, Fluorogenic

Cyclic fluorogenic substrate for the quantitative determination of calpain -1 and -2.

• Sinónimos: Calpain Substrate II, Fluorogenic, Suc-LY-AMC
• Fórmula empírica (notación de Hill): C₂₉H₃₁N₃O₇
• Número de CAS: 94367-20-1
• Peso molecular: 533.57
• Número MDL: MFCD00057900
• Código UNSPSC: 12352200
• NACRES: NA.77

Propiedades

• Nivel de calidad: 100
• Análisis: ≥95% (TLC)
• formulario: solid
• fabricante / nombre comercial: Calbiochem^®
• condiciones de almacenamiento: OK to freeze; desiccated; protect from light
• color: off-white
• solubilidad: DMSO: soluble
• fluorescencia: λ_ex ~380 nm; λ_em ~460 nm
• Condiciones de envío: ambient
• temp. de almacenamiento: −20°C
• InChI: 1S/C29H33N3O8/c1-4-16(2)27(32-24(34)11-12-25(35)36)29(39)31-22(14-18-5-8-20(33)9-6-18)28(38)30-19-7-10-21-17(3)13-26(37)40-23(21)15-19/h5-10,13,15-16,22,27,33H,4,11-12,14H2,1-3H3,(H,30,38)(H,31,39)(H,32,34)(H,35,36)/t16?,22?,27-/m0/s1
• Clave InChI: VSWNAJAOJZXWBM-FTJGSZSVSA-N

Descripción

Descripción general

Cyclic fluorogenic substrate for the quantitative determination of calpain -1 and -2. Also suitable for measuring the peptidase activity of the 20S proteasome. Typical concentrations range from 10 - 100 µM, depending on assay conditions.

Fluorogenic substrate for the quantitative determination of porcine calpain I and II activity. Also serves as a substrate for other proteases including proteasome. (excitation wavelength: 380 nm; emission wavelength: 460 nm)

Acciones bioquímicas o fisiológicas

Cell permeable: no

Primary Target
Calpain -1 and -2

Product does not compete with ATP.

Reversible: no

Advertencia

Toxicity: Standard Handling (A)

Secuencia

Suc-Leu-Tyr-AMC

Reconstitución

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.

Otras notas

Seol, J.H., et al. 1989. FEBS Lett. 247, 197.
Woo, K.M., et al. 1989. J. Biol. Chem. 264, 2088.
Sasaki, T., et al. 1984. J. Biol. Chem.259, 12489.

Información legal

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Información de seguridad

• Código de clase de almacenamiento: 11 - Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 3
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable