197217

Bcl-x_L BH4₄₋₂₃, Human, Cell-Permeable

A cell-permeable peptide that prevents apoptotic cell death by directly binding to the voltage-dependent anion channel (VDAC) and blocking its activity.

• Sinónimos: Bcl-x_L BH4₄₋₂₃, Human, Cell-Permeable, HIV-TAT₄₈₋₅₇-β-Ala-Bcl-x _L BH4₄₋₂₃, Ac-GRKKRRQRRR-βA-SNRELVVDFLSYKLSQKGYS-OH
• Fórmula empírica (notación de Hill): C₁₆₅H₂₇₉N₅₉O₄₆
• Peso molecular: 3825.35
• Código UNSPSC: 12352202
• NACRES: NA.77

Propiedades

• Nivel de calidad: 100
• Ensayo: ≥95% (HPLC)
• Formulario: lyophilized
• fabricante / nombre comercial: Calbiochem^®
• condiciones de almacenamiento: OK to freeze; desiccated (hygroscopic)
• solubilidad: 20% acetic acid: 5 mg/mL; DMSO: 5 mg/mL
• Condiciones de envío: ambient
• temp. de almacenamiento: −20°C

Descripción

Descripción general

A cell-permeable peptide that prevents apoptotic cell death by directly binding to the voltage-dependent anion channel (VDAC) and blocking its activity. Leads to the inhibition of cytochrome c release and loss of mitochondrial membrane potential (ΔΨ_m). Contains the conserved N-terminal homology domain (BH4) of Bcl-x_L (amino acids 4 - 23) that has been shown to be essential for inhibiting VDAC activity in liposomes and in isolated mitochondria. The BH4 domain is linked to a carrier peptide, a 10-amino acid HIV-TAT_48-57 sequence with a β-alanine residue as a spacer for maximum flexibility. Following its uptake, it is mainly localized to the mitochondria.

A highly cell-permeable peptide that prevents apoptotic cell death by directly binding to the voltage-dependent anion channel (VDAC) and blocking its activity. Ultimately results in the inhibition of cytochrome c release and loss of mitochondrial membrane potential (ΔΨm). Contains the conserved N-terminal homology domain (BH4) of Bcl-x_L (amino acids 4-23) that has been shown to be essential for inhibiting VDAC activity in liposomes and isolated mitochondria. The BH4 domain is linked to the carrier peptide, the 10-amino acid HIV-TAT_48-57 sequence with a β-alanine residue as a spacer for maximum flexibility. Following its uptake, it is mainly localized in the mitochondria.

Acciones bioquímicas o fisiológicas

Cell permeable: yes

Primary Target
Voltage-dependent anion channel (VDAC)

Product does not compete with ATP.

Reversible: yes

Envase

Packaged under inert gas

Advertencia

Toxicity: Standard Handling (A)

Secuencia

Ac-Gly-Arg-Lys-Lys-Arg-Arg-Gln-Arg-Arg-Arg-βAla-Ser-Asn-Arg-Glu-Leu-Val-Val-Asp-Phe-Leu-Ser-Tyr-Lys-Ser-Gln-Lys-Gly-Tyr-Ser-OH

Forma física

Supplied as a trifluoroacetate salt.

Reconstitución

Following reconstitution aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Otras notas

Chen m., et al. 2002. J. Biol. Chem. 277, 29181.
Futaki, S., et al. 2001. J. Biol. Chem.276, 5836.
Shimizu, S., et al. 2000. Proc. Natl. Acad. Sci. USA97, 3100.

Información legal

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Información de seguridad

• Código de clase de almacenamiento: 11 - Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable