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Merck
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Key Documents

16-316

Sigma-Aldrich

Anti-Phosphotyrosine Antibody, 4G10® Platinum, HRP Conjugate

Upstate®, from mouse, peroxidase conjugate

Sinónimos:

phosphotyrosine

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About This Item

Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

origen biológico

mouse

Nivel de calidad

conjugado

peroxidase conjugate

tipo de anticuerpo

primary antibodies

clon

4G10®, monoclonal
PY20, monoclonal

reactividad de especies (predicha por homología)

all

fabricante / nombre comercial

Upstate®

técnicas

ELISA: suitable
western blot: suitable

isotipo

IgG2b

Condiciones de envío

wet ice

modificación del objetivo postraduccional

phosphorylation (pTyr)

Información sobre el gen

human ... PID1(55022)

Descripción general

The development of the anti-phosphotyrosine, clone 4G10 in 1989 was a monumental discovery for researchers. 4G10 was the first and is the best single monoclonal antibody for the detection tyrosine phosphorylation. 4G10 is well known for its sensitivity and its ability to detect multiple tyrosine phosphorylations on numerous substrates. It has been validated by thousands of scientific and medical researchers in virtually every application and tyrosine target over the past 2 decades. To improve on something that hundred have tried and no one has succeeded, we pooled 4G10 with the next most highly regarded anti-phosphotyrosine, clone PY20 to make 4G10 Platinum. PY20 itself is a very poor substitute for 4G10, but its additive effect allow for a greater level of detection on more substrates that even 4G10 alone.

Aplicación

Detect Phosphotyrosine with Anti-Phosphotyrosine Antibody, 4G10 Platinum, HRP Conjugate (Mouse Monoclonal Antibody), that has been shown to work in WB, ELISA.

Calidad

Routinely evaluated by western blot.

Descripción de destino

Varies

Forma física

100 μL of a proprietary mixture of protein G purified mouse monoclonal 4G10 (IgG2bƙ) HRP conjugate and protein A purified mouse monoclonal PY20, HRP conjugate (IgG2b) in PBS containing 0.05% Kathon and HRP stabilizing compounds. Sufficient for a minumum of 10 immunoblots, if used at the optimum concentration of 1:2000 – 1:4000 in 10 mL blocking buffer. Liquid.

Información legal

4G10 is a registered trademark of Upstate Group, Inc.
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

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Clasificaciones de peligro

Aquatic Chronic 2 - Skin Sens. 1

Clase de riesgo para el agua (WGK)

WGK 3

Punto de inflamabilidad (°F)

does not flash

Punto de inflamabilidad (°C)

does not flash


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Ondřej Ballek et al.
Immunology letters, 142(1-2), 64-74 (2012-01-28)
Lck is the principal signal-generating tyrosine kinase of the T cell activation mechanism. We have previously demonstrated that induced Lck activation outside of lipid rafts (LR) results in the rapid translocation of a fraction of Lck to LR. While this
Irmgard Hofmann et al.
The Journal of pharmacology and experimental therapeutics (2022-10-15)
VEGF and angiopoietin-2 (ANG2) have complementary roles in angiogenesis and promote an immunosuppressive tumor microenvironment. It is anticipated that combination of VEGF and ANG2 blockade could provide superior activity to blockade of either pathway alone, and that addition of VEGF/ANG2
Wei Tian et al.
BMC biochemistry, 13, 22-22 (2012-10-25)
Phosphatase of regenerating liver-3 (PRL-3 or PTP4A3) has been implicated in controlling cancer cell proliferation, motility, metastasis, and angiogenesis. Deregulated expression of PRL-3 is highly correlated with cancer progression and predicts poor survival. Although PRL-3 was categorized as a tyrosine
Pierluigi Scalia et al.
Oncogene, 38(31), 5987-6001 (2019-07-05)
Malignant mesothelioma is a deadly disease with limited therapeutic options. EphB4 is an oncogenic tyrosine kinase receptor expressed in malignant mesothelioma as well as in a variety of cancers. It is involved in tumor microenvironment mediating angiogenesis and invasive cellular
Tariq S Adwan et al.
The Journal of biological chemistry, 286(41), 35716-35724 (2011-08-26)
PKCδ translocates into the nucleus in response to apoptotic agents and functions as a potent cell death signal. Cytoplasmic retention of PKCδ and its transport into the nucleus are essential for cell homeostasis, but how these processes are regulated is

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