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16-104

Sigma-Aldrich

Anti-Phosphotyrosine Antibody, clone 4G10®, FITC conjugate

clone 4G10®, Upstate®, from mouse

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About This Item

Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

origen biológico

mouse

Nivel de calidad

conjugado

FITC conjugate

tipo de anticuerpo

primary antibodies

clon

4G10®, monoclonal

reactividad de especies (predicha por homología)

all

fabricante / nombre comercial

Upstate®

técnicas

immunocytochemistry: suitable

Condiciones de envío

wet ice

modificación del objetivo postraduccional

phosphorylation (pTyr)

Información sobre el gen

human ... PID1(55022)

Descripción general

Anti-phosphotyrosine antibody (05-321), cross-linked to fluorescein isothiocyanate (FITC).

Especificidad

Recognizes tyrosine-phosphorylated proteins from all species.

Inmunógeno

Phosphotyramine-KLH.

Aplicación

Detect Phosphotyrosine using this Anti-Phosphotyrosine Antibody, clone 4G10, FITC conjugate validated for use in IC.
For use in applications in which a FITC conjugate is advantageous, such as ICC.
Research Category
Signaling
Research Sub Category
General Post-translation Modification

Calidad

routinely evaluated in immunocytochemistry to detect tyrosine phosphorylated proteins in EGF-stimulated human A431 cells

Forma física

PBS containing 0.05% sodium azide
Protein G Purified

Almacenamiento y estabilidad

Stable for 9 months at 4°C

Nota de análisis

Control
Pervanadate-treated human A431 cell extracts or EGF-treated human A431 cells

Información legal

4G10 is a registered trademark of Upstate Group, Inc.
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Código de clase de almacenamiento

12 - Non Combustible Liquids

Clase de riesgo para el agua (WGK)

WGK 2

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable


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Xiaomin Zhang et al.
Neuron, 107(6), 1212-1225 (2020-08-09)
Dentate gyrus granule cells (GCs) connect the entorhinal cortex to the hippocampal CA3 region, but how they process spatial information remains enigmatic. To examine the role of GCs in spatial coding, we measured excitatory postsynaptic potentials (EPSPs) and action potentials
Experimental inoculation of juvenile rhesus macaques with primate enteric caliciviruses.
Sestak, K; Feely, S; Fey, B; Dufour, J; Hargitt, E; Alvarez, X; Pahar, B; Gregoricus et al.
Testing null
Kelly L Asquith et al.
Journal of cell science, 117(Pt 16), 3645-3657 (2004-07-15)
Mammalian spermatozoa undergo a series of molecular and biochemical changes collectively termed capacitation prior to acquiring the ability to fertilise the oocyte. Although phosphorylation of sperm proteins on tyrosine residues has been recognised as an important component of this process
Carolina Borges-Merjane et al.
Neuron, 105(6), 992-1006 (2020-01-14)
How structural and functional properties of synapses relate to each other is a fundamental question in neuroscience. Electrophysiology has elucidated mechanisms of synaptic transmission, and electron microscopy (EM) has provided insight into morphological properties of synapses. Here we describe an
Stefano De Tito et al.
Methods in molecular biology (Clifton, N.J.), 2557, 225-234 (2022-12-14)
The Golgi apparatus is a highly dynamic organelle that controls lipid and protein sorting in the endocytic and exocytic cellular pathways. Perturbation of the lipid homeostasis or of the molecular machineries that regulate membrane remodeling/trafficking events on the Golgi membranes

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