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Supelco

SeQuant ZIC®-HILIC 200Å (3.5 µm) HPLC Columns

L × I.D. 150 mm x 4.6 mm HPLC Column, PEEK-lined stainless steel

Sinónimos:

Zwitterionic Hydrophilic Interaction Liquid Chromatography HPLC Column

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About This Item

Código UNSPSC:
41115709

product name

SeQuant ZIC-HILIC 3.5µm,200Å 150 x 4.6 mm, PEEK-lined stainless steel column, suitable for HPLC

Materiales

PEEK-lined stainless steel column

Nivel de calidad

Agency

suitable for USP L114

Línea del producto

SeQuant

Características

endcapped: no

Parámetros

500 bar pressure
70 °C max. temp.

técnicas

HPLC: suitable
LC/MS: suitable
mass spectrometry (MS): suitable (High)

columna L × D.I.

15 cm × 4.6 mm

matriz

fully porous particle

grupo activo de la matriz

ZIC-HILIC bonding phase

tamaño de partícula

3.5 μm

tamaño de poro

200 Å pore size

pH operativo

2-7.5

técnica de separación

hydrophilic interaction (HILIC)

temp. de almacenamiento

no temp limit

Descripción general

SeQuant ZIC®-HILIC Columns are designed to retain and separate all types of polar and hydrophilic compounds and for robust chromatography with high selectivity and reproducibility.
The silica-based ZIC®-HILIC sorbent has a bonded stationary phase consisting of a highly polar, permanent zwitterion. Separation selectivity is favored by the 1:1 zwitterion charge balance, which makes the ZIC®-HILIC Column overall neutral, with weak, but important, ionic interactions. Tuning of the selectivity on the ZIC®-HILIC Column during method development is facilitated by the pH-inpendent permanent zwitterion, ensuring that only the analytes (and not the column) are affected during eluent optimization.
The Pore size of 200 Å makes this column perfectly suitable for the separation of larger polar molecules under HILIC conditions.
The column dimension of 250 mm length x 2.1 mm ID is the optimal choice for the separation of complex samples using a UHPLC or optimized HPLC Instrument providing low dwell volumes.
Thanks to their favorable retention and robust performance, SeQuant HILIC columns have become extremely popular in numerous laboratories around the world. For example, US FDA (United States Food and Drug Administration) has recommended SeQuant ZIC®-HILIC for analysis of melamine and related substances. SeQuant HILIC columns excel in stability and low bleed. These features make them particularly suitable for LC-MS applications where high background can lead to signal suppression and interference with quantitative measurements while also increasing instrument wear. Their high stability makes these high-performance HILIC columns also attractive for traditional HPLC applications.
Learn more about the Supelco HPLC Column portfolio and Method development:
Brochure: HPLC and UHPLC Column Selection Guide
Brochure: A Practical Guide to HPLC - Method Development Guide

Información legal

SeQuant is a trademark of Merck KGaA, Darmstadt, Germany
ZIC is a registered trademark of Merck KGaA, Darmstadt, Germany
ZIC-HILIC is a trademark of Merck KGaA, Darmstadt, Germany

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