14-613-M

Brk Protein, active, 10 µg

• Código UNSPSC: 12352202
• eCl@ss: 32160405
• NACRES: NA.32

Propiedades

• recombinante: expressed in baculovirus infected Sf21 cells
• Nivel de calidad: 300
• descripción: protein target BRK
• purificado por: (Ni²⁺/NTA-agarose)
• técnicas: activity assay: suitable
• Nº de acceso UniProt: Q13882
• Información sobre el gen: human ... PTK6(5753)

Descripción

Descripción general

FUNCTION: SwissProt: Q13882 # Phosphorylates KHDRBS1, KHDRBS2, KHDRBS3 and STAP2/BKS. May function as an intracellular signal transducer in epithelial tissues. Overexpression in mammary cells leads to mitogenically sensitization to EGF, and results in a partially transformed phenotype. Its presence in the nucleus appears to be linked to suppression of tumor progression.
SIZE: 451 amino acids; 51834 Da
SUBUNIT: Interacts with GAP-A.p65 (By similarity). Interacts with KHDRBS1.
SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Note=Colocalizes with KHDRBS1, KHDRBS2 or KHDRBS3, within the nucleus. In secretory epithelial cells from prostate adenocarcinoma, nuclear localization is higher in low-grade and lower in high-grade regions of the tumors.
TISSUE SPECIFICITY: Epithelia-specific. Very high level in colon and high levels in small intestine and prostate, and low levels in some fetal tissues. Expressed at low level in some breast tumors, but not in normal breast. Also found in melanocytes. Not expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.
PTM: Autophosphorylated. The phosphorylation of Tyr-447 may lead to the autoinhibition of the enzyme.
SIMILARITY: SwissProt: Q13882 ## Belongs to the protein kinase superfamily. Tyr protein kinase family. BRK/PTK6/SIK subfamily. & Contains 1 protein kinase domain. & Contains 1 SH2 domain. & Contains 1 SH3 domain.

Envase

Also available in 10 μg size (cat#14-613) and in bulk (cat#14-613-K).

Calidad

Routinely evaluated by phosphorylation of Poly (Glu4-Tyr)

routinely evaluated by phosphorylation of Poly (Glu4-Tyr)

Propiedades físicas

55.3kDa

Almacenamiento y estabilidad

6 months at -70°C

Nota de análisis

For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.