1.25079

Fractogel^® EMD DEAE (M)

MiniChrom Fractogel^® DEAE (M), volume 5 mL, prepacked column

• Sinónimos: MiniChrom Column Fractogel^® DEAE (M), 5 ml
• Código UNSPSC: 41115711

Propiedades

• ligand: diethyl-(2-hydroxypropyl)aminoethyl
• Materiales: polypropylene housing
• Línea del producto: Fractogel^®
• formulario: prepacked column
• Parámetros: ≤8 bar pressure; ~170 cm/hr max. flow rate
• cama L: 100 mm
• lecho volumen: 5 mL
• columna D.I.: 8 mm
• volumen: 5 mL
• grupo activo de la matriz: Methacrylate
• tamaño medio de partícula: 40-90 μm
• capacidad: ~100 mg, BSA binding capacity
• aplicaciones: gene therapy; mAb; vaccine development
• técnica de separación: anion exchange
• temp. de almacenamiento: 15-25°C

Descripción

Descripción general

Weak cation exchange Fractogel^® EMD DEAE (M) resin in a convenient pre-packed 5 ml column

Características y beneficios

MiniChrom Column Fractogel^® DEAE (M), 5 ml enables:

• Excellent binding to large viruses and plasmid DNA
• Homogenous binding with high selectivity and purity
• Lower elution volumes for the highest purity levels
• Compatibility with 2.5 % (v/v) aqueous benzyl alcohol containing 150 mM NaCl storage solution

Due to the titration behavior, the ion exchange capacity can be used from pH 2 to pH 9.5. The separation of proteins is based on reversible electrostatic interactions between the negatively charged regions of the proteins′ surface and the support. Proteins are retained efficiently on Fractogel^® EMD DEAE when the pH of the buffer is about 1 unit above their isoelectric points (pl).

The strength of the binding depends on the following:

• the buffer system
• pH value of the buffer which determines the surface charge of the protein
• the degree of the ionization of the functional groups of the exchanger
• the concentration of the counter ions
• the charge density on the support (protein binding capacity)

Información legal

FRACTOGEL is a registered trademark of Merck KGaA, Darmstadt, Germany