1.15943

Methylene blue (C.I.52015)

for microscopy Certistain^®

• Sinónimos: Methylene Blue trihydrate, Methylthioninium chloride, 3,7-Bis(dimethylamino)phenothiazinium chloride, Tetramethylthionine chloride
• Fórmula empírica (notación de Hill): C₁₆H₁₈ClN₃S · 3H₂O
• Número de CAS: 7220-79-3
• Peso molecular: 373.90
• Beilstein: 3599847
• Número MDL: MFCD00012111
• Código UNSPSC: 41116130
• Número índice de la CE: 200-515-2

Propiedades

• Nivel de calidad: 400
• formulario: solid
• potencia: 1180 mg/kg LD₅₀, oral (Rat)
• composición: Dye, ≥82% spectrophotometric assay
• pH: 3 (20 °C, 10 g/L in H₂O)
• mp: 190 °C (dec.) (lit.)
• solubilidad: 50 g/L
• densidad: 1.757 g/cm³ at 22 °C
• densidad aparente: 400‑600 kg/m³
• εmax: 2250-2750 at 660-665 nm in 50% ethanol at 0.003 g/L
• aplicaciones: diagnostic assay manufacturing; hematology; histology
• temp. de almacenamiento: 2-30°C
• cadena SMILES: [Cl-].CN(C)c1ccc2N=C3C=C\C(C=C3Sc2c1)=[N+](/C)C
• InChI: 1S/C16H18N3S.ClH/c1-18(2)11-5-7-13-15(9-11)20-16-10-12(19(3)4)6-8-14(16)17-13;/h5-10H,1-4H3;1H/q+1;/p-1
• Clave InChI: CXKWCBBOMKCUKX-UHFFFAOYSA-M

Descripción

Descripción general

Methylene blue (C.I. 52015) for microscopy Certistain^® is a dry dye that is used in bacteriology for an overview stain and as counterstaining for Ziehl-Neelsen method in sample material of human origin. Bacteria appear blue. The dye has a further important application in hematology: Methylene blue and eosin are the main components of many staining solutions and dye mixtures. Certistain^® is the product group for seleceted high quality dyes that fulfill all quality requirements of IVD and CE registration. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.

Nota de análisis

Dye content (spectrophotometrically): ≥ 82 %
Identity
- Chloride:
passes test
- UV/VIS-Spectrum: passes test
Absorption maximum λ max (ethanol 50 %): 660 - 665 nm
Spec. Absorptivity A 1%/1cm (λ_max; 0.003 g/l; ethanol 50 %): 2250 - 2750
TLC-Test: passes test
Loss on drying (110 °C): 10 - 15 %
Suitability for microscopy: passes test

Información legal

CERTISTAIN is a registered trademark of Merck KGaA, Darmstadt, Germany

Información de seguridad

• Pictogramas: GHS07
• Palabra de señalización: Warning
• Frases de peligro: H302
• Consejos de prudencia: P264 - P270 - P301 + P312 - P501
• Clasificaciones de peligro: Acute Tox. 4 Oral
• Código de clase de almacenamiento: 11 - Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 3
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable