1.02442

Cloroformo

for analysis (for determinations with dithizone)

• Sinónimos: Triclorometano, Tricloruro de metilidino
• Fórmula empírica (notación de Hill): CHCl₃
• Número de CAS: 67-66-3
• Peso molecular: 119.38
• Beilstein: 1731042
• Número MDL: MFCD00000826
• Código UNSPSC: 12191502
• Número índice de la CE: 200-663-8
• NACRES: NA.21

Propiedades

• densidad de vapor: 4.1 (vs air)
• Nivel de calidad: 300
• presión de vapor: 160 mmHg ( 20 °C)
• Análisis: 99.0-99.4% (GC)
• formulario: liquid
• potencia: 695 mg/kg LD₅₀, oral (Rat); >3980 mg/kg LD₅₀, skin (Rabbit)
• impurezas: ≤0.0001% Chloride (Cl); ≤0.001% Free acid (as HCl); ≤0.005% Carbonyl compounds (as CO); ≤0.01% Carbon tetrachloride (GC); ≤0.01% Dichloromethane (GC); ≤0.01% Tetrachloroethylene (GC); ≤0.01% Trichloroethylene (GC); ≤0.01% Water; 0.6-1% Ethanol (GC); 0.6-1.0% Ethanol (GC)
• residuo de evap.: ≤0.001%
• índice de refracción: n20/D 1.445 (lit.)
• bp: 60.5-61.5 °C (lit.)
• mp: −63 °C (lit.)
• densidad: 1.492 g/mL at 25 °C (lit.)
• trazas de catión: Al: ≤0.00005%; B: ≤0.000002%; Ba: ≤0.00001%; Ca: ≤0.00005%; Cd: ≤0.000005%; Co: ≤0.000002%; Cr: ≤0.000002%; Cu: ≤0.000002%; Fe: ≤0.00001%; Mg: ≤0.00001%; Mn: ≤0.000002%; Ni: ≤0.000002%; Pb: ≤0.00001%; Sn: ≤0.00001%; Zn: ≤0.00001%
• temp. de almacenamiento: 2-30°C
• cadena SMILES: ClC(Cl)Cl
• InChI: 1S/CHCl3/c2-1(3)4/h1H
• Clave InChI: HEDRZPFGACZZDS-UHFFFAOYSA-N

Descripción

Aplicación

• Characterization of Natural Products: Chloroform is extensively used in the extraction and isolation of natural products. A study on the characterization and pesticidal evaluation of diterpenes isolated from chloroform extracts of Hedychium spicatum rhizomes highlights its effectiveness. This application is crucial for identifying bioactive compounds and assessing their potential uses in pharmaceuticals and agrochemicals (Rawat et al., Analytical Chemistry Letters, 2023).


• Self-Diffusion and Molecular Association Studies: Chloroform is used in studies of self-diffusion and molecular association in binary systems, such as dimethyl sulfoxide (DMSO) and acetone. These studies provide insights into the molecular interactions and dynamics in mixed solvents, which are essential for understanding solvation and reaction mechanisms in various chemical processes (Gurina and Golubev, Results in Chemistry, 2022).


• Cell-Free DNA Isolation: A novel phenol-chloroform-free method for isolating cell-free DNA from cell-conditioned media has been developed, optimized, and compared with traditional methods. This advancement is significant for genomic studies, allowing for more efficient and less hazardous extraction of nucleic acids from biological samples (Das et al., Analytical Biochemistry, 2024).


• Green Chemistry and Circular Economy: Chloroform plays a role in green analytical chemistry, particularly in the development of circular chemistry principles. Research emphasizes the need to design chemical processes that minimize waste and environmental impact, promoting sustainability in analytical laboratories. This includes the use of chloroform in recyclable and reusable systems to reduce solvent waste (Psillakis and Pena-Pereira, TrAC Trends in Analytical Chemistry, 2024).

Nota de análisis

Purity (GC): 99.0 - 99.4 %
Identity (IR): conforms
Free acid (as HCl): ≤ 0.001 %
Chloride (Cl): ≤ 0.0001 %
Free chlorine (Cl): conforms
Carbonyl compounds (as CO): ≤ 0.005 %
Matter discolored by H₂SO₄: ≤ 10 Hazen
Ethanol (GC): 0.6 - 1.0 %
Dichloromethane (GC): ≤ 0.01 %
Carbon tetrachloride (GC): ≤ 0.01 %
Tetrachloroethylene (GC): ≤ 0.01 %
Trichloroethylene (GC): ≤ 0.01 %
Evaporation residue: ≤ 0.001 %
Water: ≤ 0.01 %
Suitability for determination with dithizone: conforms
Al (Aluminium): ≤ 0.00005 %
B (Boron): ≤ 0.000002 %
Ba (Barium): ≤ 0.00001 %
Ca (Calcium): ≤ 0.00005 %
Cd (Cadmium): ≤ 0.000005 %
Co (Cobalt): ≤ 0.000002 %
Cr (Chromium): ≤ 0.000002 %
Cu (Copper): ≤ 0.000002 %
Fe (Iron): ≤ 0.00001 %
Mg (Magnesium): ≤ 0.00001 %
Mn (Manganese): ≤ 0.000002 %
Ni (Nickel): ≤ 0.000002 %
Pb (Lead): ≤ 0.00001 %
Sn (Tin): ≤ 0.00001 %
Zn (Zinc): ≤ 0.00001 %
Stabilized with 0.6 - 1.0% ethanol.

Información de seguridad

• Pictogramas: GHS06,GHS08
• Palabra de señalización: Danger
• Frases de peligro: H302 - H315 - H319 - H331 - H336 - H351 - H361d - H372
• Consejos de prudencia: P202 - P301 + P312 - P302 + P352 - P304 + P340 + P311 - P305 + P351 + P338 - P308 + P313
• Clasificaciones de peligro: Acute Tox. 3 Inhalation - Acute Tox. 4 Oral - Carc. 2 - Eye Irrit. 2 - Repr. 2 - Skin Irrit. 2 - STOT RE 1 Oral - STOT SE 3
• Órganos de actuación: Central nervous system
• Código de clase de almacenamiento: 6.1D - Non-combustible, acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects
• Clase de riesgo para el agua (WGK): WGK 3
• Punto de inflamabilidad (°F): does not flash
• Punto de inflamabilidad (°C): does not flash