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Merck
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Key Documents

1.00327

Sigma-Aldrich

Hydrogen chloride solution

in ethanol, for microscopy

Sinónimos:

Hydrogen chloride – ethanol solution, Hydrogen chloride solution, Hydrochloric acid in ethanol

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About This Item

Fórmula lineal:
HCl · CH3CH2OH
Número de CAS:
Peso molecular:
36.46
Beilstein:
1098214
Número MDL:
Código UNSPSC:
41116130
NACRES:
NA.21

product name

Hydrogen chloride solution, in ethanol, for microscopy

Nivel de calidad

formulario

liquid

DIV

for in vitro diagnostic use

pH

1.0 (20 °C in H2O)

temperatura de transición

flash point 21 °C

densidad

0.90 g/cm3 at 20 °C

aplicaciones

clinical testing
diagnostic assay manufacturing
hematology
histology

temp. de almacenamiento

2-30°C

cadena SMILES

Cl

InChI

1S/ClH/h1H

Clave InChI

VEXZGXHMUGYJMC-UHFFFAOYSA-N

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Descripción general

Hydrochloric acid in ethanol - for microscopy, is used for human-medical cell diagnosis and serves the purpose of the bacteriological and histological investigation of sample material of human origin. Acid-fast bacteria are difficult to stain because of the high proportion of lipid and wax in their cell walls. The most efficient staining method is the hot staining according to Ziehl-Neelsen. In this method, carbolfuchsin solution is applied to the specimen and heated. This heating process accelerates the rate at which the fuchsin dye is absorbed and thus also that of the formation of the mycolatefuchsin complex in the cell wall. With the Kinyoun method, a modification of the AFB-Color carbol fuchsin solution renders heating unnecessary (cold staining). Once the acid-fast bacteria have absorbed the fuchsin dye, it is virtually impossible to decolorize them again, even when they are intensively treated with a decolorizing solution such as e. g. hydrochloric acid in ethanol (Product number 1.00327). Accordingly, acid-fast bacteria are termed as acid- and alcohol-fast for staining, and are stained red in the microscopic visualization. Correspondingly, all non-acid-fast microorganisms are counterstained with an appropriate dye. In this instruction for use malachite green is used for counterstaining.
Pretreatment of the specimens with Sputofluol dissolves the bacteria from the surrounding viscid sputum and cell material. It is an IVD and CE registered product and can be applied for diagnostical purposes. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.

Aplicación

For microscopy. Ziehl-Neelsen staining

Información legal

SPUTOFLUOL is a trademark of Merck KGaA, Darmstadt, Germany

Pictogramas

FlameCorrosion

Palabra de señalización

Danger

Frases de peligro

Clasificaciones de peligro

Eye Irrit. 2 - Flam. Liq. 2 - Met. Corr. 1

Código de clase de almacenamiento

3 - Flammable liquids

Clase de riesgo para el agua (WGK)

WGK 1

Punto de inflamabilidad (°F)

69.8 °F

Punto de inflamabilidad (°C)

21 °C


Certificados de análisis (COA)

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Contenido relacionado

An overview of the science and practice of bacteriology in clinical diagnostics. Learn more about the application of standard and special stains for microscopic analysis.

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