07-595

Anti-acetyl-Histone H4 (Lys12) Antibody

serum, Upstate^®

• Sinónimos: H4K12Ac, Histone H4 (acetyl K12)
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propiedades

• origen biológico: rabbit
• Nivel de calidad: 100
• forma del anticuerpo: serum
• tipo de anticuerpo: primary antibodies
• clon: polyclonal
• reactividad de especies: Saccharomyces cerevisiae, human
• fabricante / nombre comercial: Upstate^®
• técnicas: ChIP: suitable (ChIP-seq); dot blot: suitable; western blot: suitable
• isotipo: IgG
• Nº de acceso NCBI: NM_175054
• Nº de acceso UniProt: P62805; Q6DRA9
• Condiciones de envío: dry ice
• modificación del objetivo postraduccional: acetylation (Lys12)
• Información sobre el gen: human ... HIST2H4B(554313)

Descripción

Descripción general

Histone H4 is one of the 5 main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N terminal tail H4 is involved with the structure of the nucleosomes of the ′beads on a string′ structure.

Acetylation of histone H4 occurs at several different lysine positions in the histone tail and is performed by a family of enzymes known as Histone Acetyl Transferases (HATs).

Especificidad

Broad species cross-reactivity expected

Recognizes Histone H4 acetylated on lysine 12.

Inmunógeno

peptide containing the sequence (LG_AcKGG) corresponding to lysine 12 acetylation of yeast Histone H4.

Aplicación

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Histones

Use Anti-acetyl-Histone H4 (Lys12) Antibody (Rabbit Polyclonal Antibody) validated in ChIP, DB, WB, ChIP-seq to detect acetyl-Histone H4 (Lys12) also known as H4K12Ac, Histone H4 (acetyl K12).

Calidad

routinely evaluated by immunoblot on acid extracted proteins from HeLa cells

Descripción de destino

10 kDa

Forma física

Depleted rabbit antiserum in 30% glycerol, 0.07M Tris-glycine, pH 7.4, 0.105 M NaCl, 0.035% sodium azide as a preservative.

Immunodepleted Serum

Almacenamiento y estabilidad

Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Nota de análisis

Control
Acid extracted proteins from HeLa cells treated with sodium butyrate

Otras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Información legal

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 10 - Combustible liquids
• Clase de riesgo para el agua (WGK): WGK 1