07-520

Anti-eNOS/NOS III Antibody, CT

Upstate^®, from rabbit

• Sinónimos: Constitutive NOS, Endothelial NOS, Endothelial NOS, Endothelial NOS, endothelial nitric oxide synthase, nitric oxide synthase 3 (endothelial cell)
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propiedades

• origen biológico: rabbit
• Nivel de calidad: 100
• forma del anticuerpo: purified immunoglobulin
• tipo de anticuerpo: primary antibodies
• clon: polyclonal
• reactividad de especies: mouse, pig, guinea pig, rat, canine, human, bovine
• fabricante / nombre comercial: Upstate^®
• técnicas: western blot: suitable
• isotipo: IgG
• Nº de acceso NCBI: NP_000594
• Nº de acceso UniProt: P29474
• Condiciones de envío: dry ice
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: bovine ... Nos3(287024) ; dog ... Nos3(403784) ; guinea pig ... Nos3(100135577) ; human ... NOS3(4846) ; mouse ... Nos3(18127) ; pig ... Nos3(397557) ; rat ... Nos3(24600)

Descripción

Descripción general

Endothelial NOS (eNOS), also known as Nitric oxide synthase 3 (NOS3) or constitutive NOS (cNOS), is a nitric oxide synthase. It generates NO in blood vessels and is involved with regulating vascular function. A constitutive Ca2+ dependent NOS provides a basal release of NO. eNOS is associated with plasma membranes surrounding cells and the membranes of Golgi bodies within cells.

Especificidad

Predicted to cross-react with mouse, guinea pig, pig, rat and dog based on sequence homology.

Recognizes eNOS/NOS III, Mr ~135 kDa.

Inmunógeno

KLH-conjugated, synthetic peptide corresponding to amino acids 1181-1194 (C-QERQLRGAVPWAFD) of human eNOS/NOS III. The immunizing sequence has 10/11 identical amino acids in mouse, guinea pig, bovine, pig, rat, and dog.

Aplicación

Anti-eNOS/NOS III Antibody, CT detects level of eNOS/NOS III & has been published & validated for use in WB.

Calidad

Routinely evaluated by western blot in RIPA lysates from HUVEC cells.

Western Blot Analysis:
0.5-2 µg/mL of this lot detected eNOS/NOS III in RIPA lysates from HUVEC cells.

Descripción de destino

135 kDa

Forma física

Format: Purified

Purified rabbit polyclonal IgG in buffer containing 0.014 M phosphate buffer, pH 7.6, 0.175 M NaCl, 0.07% sodium azide before the addition of glycerol to 30%. Liquid at -20°C.

Nota de análisis

Control
Platelets, placenta, liver and kidney.

Otras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Información legal

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Información de seguridad

• Código de clase de almacenamiento: 10 - Combustible liquids
• Clase de riesgo para el agua (WGK): WGK 1