07-260
Anti-AUF1 Antibody
Upstate®, from rabbit
Sinónimos:
Anti-AUF1, Anti-AUF1A, Anti-HNRPD, Anti-P37, Anti-hnRNPD0
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About This Item
Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Productos recomendados
origen biológico
rabbit
Nivel de calidad
forma del anticuerpo
purified immunoglobulin
tipo de anticuerpo
primary antibodies
clon
polyclonal
reactividad de especies
human, mouse, rat
fabricante / nombre comercial
Upstate®
técnicas
western blot: suitable
isotipo
IgG
Nº de acceso NCBI
Nº de acceso UniProt
Condiciones de envío
dry ice
modificación del objetivo postraduccional
unmodified
Información sobre el gen
human ... HNRNPD(3184)
mouse ... Hnrnpd(11991)
rat ... Hnrnpd(79256)
Descripción general
A+U Rich RNA Binding Factor (AUF1) is comprised of four isoforms of 37, 40, 42, and 45 kDa. Although the role of each isoform has yet to be fully characterized, a direct correlation has been observed between each AUF1 isoform’s binding affinity and its RNA destabilizing activity toward different AREs (A + U rich element in the 3’ untranslated region), with the isoforms p37 and p42 being the most effective.
AUF1 is a bcl2 mRNA binding protein and potentially all its isoforms are able to form complexes with the bcl2 ARE. ARE mediated bcl2 mRNA downregulation during apoptosis involves AUF1 and suggest different roles for its four isoforms.
AUF1 is a bcl2 mRNA binding protein and potentially all its isoforms are able to form complexes with the bcl2 ARE. ARE mediated bcl2 mRNA downregulation during apoptosis involves AUF1 and suggest different roles for its four isoforms.
Especificidad
Recognizes AUF1 isoforms.
Inmunógeno
Purified AUF1
Aplicación
Anti-AUF1 Antibody is a high quality Rabbit Polyclonal Antibody for the detection of AUF1 & has been validated in WB.
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Transcription Factors
RNA Binding Protein (RBP)
Transcription Factors
RNA Binding Protein (RBP)
Calidad
routinely evaluated by immunoblot on RIPA lysates from HeLa nuclear extract or A431 cells
Descripción de destino
37, 40, 42, 45 kDa
Forma física
Format: Purified
Protein A Purified immunoglobulin in 30% glycerol, 0.07M Tris-glycine, pH 7.4, 0.105 M NaCl, 0.035% sodium azide as a preservative.
Protein A purified
Almacenamiento y estabilidad
Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Nota de análisis
Control
Positive Antigen Control: Catalog #12-309, Hela cell nuclear extract. Add an equal volume of Laemmli reducing sample buffer to 10 μL of extract and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced extract per lane for minigels.
Positive Antigen Control: Catalog #12-309, Hela cell nuclear extract. Add an equal volume of Laemmli reducing sample buffer to 10 μL of extract and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced extract per lane for minigels.
Otras notas
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Información legal
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Cláusula de descargo de responsabilidad
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Código de clase de almacenamiento
10 - Combustible liquids
Clase de riesgo para el agua (WGK)
WGK 1
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
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Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.
Destabilization of interleukin-6 mRNA requires a putative RNA stem-loop structure, an AU-rich element, and the RNA-binding protein AUF1.
Paschoud, S; Dogar, AM; Kuntz, C; Grisoni-Neupert, B; Richman, L; Kuhn, LC
Molecular and cellular biology null
Cigarette smoke enhances human rhinovirus-induced CXCL8 production via HuR-mediated mRNA stabilization in human airway epithelial cells.
Hudy, MH; Proud, D
Respiratory Research null
Steven J Cok et al.
The Journal of biological chemistry, 279(9), 8196-8205 (2003-12-10)
RAW 264.7 cells rapidly induce cyclooxygenase-2 (COX-2) in response to lipopolysaccharide treatment. Part of the increased COX-2 expression occurred through post-transcriptional mechanisms mediated through specific regions of the 3'-untranslated region (UTR) of the message. The proximal region of the 3'-UTR
The role of HuR in the post-transcriptional regulation of interleukin-3 in T cells.
Gonzalez-Feliciano, JA; Hernandez-Perez, M; Estrella, LA; Colon-Lopez, DD; Lopez et al.
Testing null
mRNA degradation plays a significant role in the program of gene expression regulated by phosphatidylinositol 3-kinase signaling.
Graham, JR; Hendershott, MC; Terragni, J; Cooper, GM
Molecular and cellular biology null
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