07-225

Anti-TrkB Antibody

Upstate^®, from rabbit

• Sinónimos: BDNF/NT-3 growth factors receptor, Neurotrophic tyrosine kinase receptor type 2, TrkB tyrosine kinase, neurotrophic tyrosine kinase receptor, type 2, tyrosine kinase receptor B
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propiedades

• origen biológico: rabbit
• forma del anticuerpo: purified antibody
• tipo de anticuerpo: primary antibodies
• clon: polyclonal
• reactividad de especies: mouse, rat
• fabricante / nombre comercial: Upstate^®
• técnicas: western blot: suitable
• isotipo: IgG
• Nº de acceso NCBI: NP_036863
• Nº de acceso UniProt: Q16620
• Condiciones de envío: dry ice
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... NTRK2(4915)

Descripción

Descripción general

The Trk (tropomyosin-receptor-kinase) proto-oncogene family of receptor tyrosine kinases contains four members, TrkA, TrkB, TrkC, and TrkE, which are variably expressed throughout the central and peripheral nervous systems. TrkB is the high-affinity to the binding brain-derived neurotrophic factor (BDNF) receptor and neurotrophin-4/5 NT-4/5). TrkB is autophosphorylated on five cytoplasmic tyrosines. The binding of BDNF to TrkB receptor causes many intercellular cascades to be activated such as promoting survival of sensory neurons mainly through Shc site-independent pathways.The signaling activity of these receptors includes the Ras – MAP kinase pathway, and the PI3 Kinase pathway. These receptors are responsible for resistance to apoptosis, obesity, Alzheimer’s disease, and mood disorders.

Especificidad

Recognizes TrkB, Mr 145 kDa, as well as an additional protein, Mr 90 kDa, which is likely a TrkB degradation product.

Inmunógeno

The entire extracellular domain (corresponding to residues 1–429) of the rat TrkB receptor, expressed in COS cells. The immunizing sequence has 97% identity with mouse TrkB and 88% identity with human TrkB.

Aplicación

Research Category
Neuroscience

Research Sub Category
Neurochemistry & Neurotrophins

This Anti-TrkB Antibody is validated for use in WB for the detection of TrkB.

Calidad

Evaluated by western blot on mouse brain membrane protein preparations.

Western Blot Analysis:
0.5-2 µg/mL of this antibody detected TrkB in mouse brain membrane protein preparations.

Descripción de destino

145 κDa

Forma física

Format: Purified

Protein A purified

Purified rabbit polyclonal in buffer containing 0.014 M phosphate buffer, pH 7.6, 0.175 M NaCl, 0.07% sodium azide, and 30% glycerol.

Almacenamiento y estabilidad

Stable for 1 year at -20ºC from date of receipt.

Nota de análisis

Control
Brain tissue.

Otras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Información legal

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 10 - Combustible liquids
• Clase de riesgo para el agua (WGK): WGK 1