07-2119

Anti-phospho-RalA Antibody (Ser194)

from rabbit, purified by affinity chromatography

• Sinónimos: Ras-related protein Ral-A
• Código UNSPSC: 12352203
• eCl@ss: 32160702

Propiedades

• origen biológico: rabbit
• Nivel de calidad: 100
• tipo de anticuerpo: primary antibodies
• clon: polyclonal
• purificado por: affinity chromatography
• reactividad de especies: human
• reactividad de especies (predicha por homología): rat (based on 100% sequence homology), zebrafish (based on 100% sequence homology), bovine (based on 100% sequence homology), Xenopus (based on 100% sequence homology)
• técnicas: inhibition assay: suitable (peptide); western blot: suitable
• Nº de acceso NCBI: NP_005393
• Nº de acceso UniProt: P11233
• Condiciones de envío: wet ice
• modificación del objetivo postraduccional: phosphorylation (pSer194)
• Información sobre el gen: human ... RALA(5898)

Descripción

Descripción general

RalA and RalB are Ras-family small monomeric GTPases that can be activated through a Ras-dependent mechanism. The two are highly homologous (89%). Ral proteins are proximal effectors of oncogenic Ras and their activity is needed to support tumorigenesis in certain cancers. Mutants of Ras that are defective in activating all known Ras effectors except Ral GEF are still able to induce invasive phenotypes in transfected cells, suggesting a role for Ral in these processes. RalA is required for anchorage-independent proliferation and tumorigeneisis by driving exocyst assembly and polarized exocytosis in epithelial cells.

Especificidad

This antibody recognizes RalA phosphorylated at Ser194.

Inmunógeno

KLH-conjugated linear peptide corresponding to human RalA phosphorylated at Ser194.

Aplicación

Detect the phospho-RalA (Ser194) protein using this Anti-phospho-RalA (Ser194) validated for use in WB & Peptide Inhibition Assay.

Western Blot Analysis: A representative lot detected phospho-RalA (Ser194) in certain primary and metastatic cell lysates, and in certain unmatched tumor tissue lysate samples.

Calidad

Evaluated by Western Blot in PANC10.05 cell lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected phospho-RalA in PANC10.05 cell lysate. Peptide inhibition was observed with the addition of phospho peptides but not with the addition of non-phospho peptides.

Descripción de destino

~25 kDa observed. Uncharacterized bands may be observed at ~20 kDa, 100 kDa, and 130-170 kDa in some cell lysates.

Nota de análisis

Control
PANC10.05 cell lysate

Otras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable