07-1512

Anti-phospho-WAVE2 (Ser343) Antibody

from rabbit, purified by affinity chromatography

• Sinónimos: SCAR2, WASF2, dJ393P12.2, WASP family protein member 2, Protein WAVE-2, Verprolin homology domain-containing protein 2
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propiedades

• origen biológico: rabbit
• Nivel de calidad: 100
• forma del anticuerpo: affinity isolated antibody
• tipo de anticuerpo: primary antibodies
• clon: polyclonal
• purificado por: affinity chromatography
• reactividad de especies: human, monkey
• técnicas: western blot: suitable
• Nº de acceso NCBI: NP_008921
• Nº de acceso UniProt: Q9Y6W5
• Condiciones de envío: wet ice
• modificación del objetivo postraduccional: phosphorylation (pSer343)
• Información sobre el gen: human ... WASF2(10163)

Descripción

Descripción general

Wiskott-Aldrich Syndrome Protein (WASP)-family Protein Member 2 (known as WAVE2) is found in many cells and tissues, with strong expression in peripheral blood leukocytes. WAVE2 is involved in signaling RTKs and small GTPases. As a whole, the WAVE family of proteins have a general function for regulating the actin cytoskeleton in various tissues.

Especificidad

This antibody recognizes WAVE2 phosphorylated at Ser343.

Inmunógeno

Epitope: Phosphorylated Ser343

KLH-conjugated linear peptide corresponding to human WAVE2 phosphorylated at Ser343.

Aplicación

Anti-phospho-WAVE2 (Ser343) Antibody detects level of phospho-WAVE2 (Ser343) & has been published & validated for use in WB.

Research Category
Cell Structure

Research Sub Category
Cytoskeletal Signaling

Western Blot Analysis: A previous lot of this antibody was used to detect endogenous phospho-WAVE2 (Ser343) in COS-7 and HMECs following EGF stimulation ± pretreatment with U0126 (Mendoza, M.C., et al. (2011). Mol Cell. 41(6):661-671).

Calidad

Evaluated by Western Blot in COS-7+EGF cell lysates untreated and lambda phosphatase-treated.

Western Blot Analysis: A 1:500 dilution of this antibody detected phospho-WAVE2 (Ser343) in 10 µg of COS-7+EGF cell lysates untreated and lambda phosphatase treated.

Descripción de destino

~75 kDa observed.
An uncharacterized band appears at ~103 kDa in some lysates.

Forma física

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Almacenamiento y estabilidad

Stable for 1 year at 2-8°C from date of receipt.

Nota de análisis

Control
COS-7+EGF cell lysates untreated and lambda phosphatase treated.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable