07-1477

Anti-Rsk1 Antibody

from rabbit, purified by affinity chromatography

• Sinónimos: 90 kDa ribosomal protein S6 kinase 1, MAP kinase-activated protein kinase 1, Ribosomal S6 kinase 1, Ribosomal protein S6 kinase alpha-1, S6K-alpha 1, p90-RSK 1, Ribosomal protein S6 kinase alpha 1, Ribosomal protein S6 kinase, 90kD, 1, Ribosmal protein S
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propiedades

• origen biológico: rabbit
• Nivel de calidad: 100
• forma del anticuerpo: purified immunoglobulin
• tipo de anticuerpo: primary antibodies
• clon: polyclonal
• purificado por: affinity chromatography
• reactividad de especies: rat, mouse, human
• técnicas: ELISA: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• isotipo: IgG
• Nº de acceso NCBI: NM_001006665.1
• Nº de acceso UniProt: Q15418
• Condiciones de envío: dry ice
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... RPS6KA1(6195)

Descripción

Descripción general

Rsk1, Rsk2, and Rsk3 (ribosomal protein S6 kinase, 90kDa, polypeptide 1,2,3; S6K-alpha 1,2,3) are Ser/Thr kinases which are activated by MAP Kinases, and are alternatively referred to as MAPKAP kinases 1a, 1b, and 1c. Following activation, Rsk isoforms translocate to the nucleus, where Rsk2 phosphorylates Ser10 of Histone H3, a critical step in the stimulation of immediate-early gene expression. Rsk1 is able to phosphorylate Ser133 of CREB, activating it as a transcription factor. Rsk1 activation can be monitored by phosphorylation state specific antibodies directed against sites involved in its activation.

Especificidad

Recognizes p90 RSK1.

Inmunógeno

Synthetic peptide corresponding to the c-terminal sequence of human p90 RSK1 (C-LAQRRVRKLPSTTL).

Aplicación

Anti-Rsk1 Antibody is an antibody against Rsk1 for use in WB, IP, ELISA.

Immunoprecipitation: 4 µg of a previous lot of this antibody was used per 1500 µg of crude protein sample in Immunoprecipitation.

ELISA: Recommended.
Optimal working dilutions must be determined by end user.

Western Blot: A previous lot of this antibody was tested on multiple lysates. See datasheet.

Calidad

Routinely evaluated by Western Blot on NIH/3T3 lysates.

Western Blot Analysis:
1:500-1:1,000 dilution of this lot detected RSK1 on 10 μg of NIH/3T3 lysates.

Descripción de destino

83 kDa

Ligadura / enlace

Replaces: 04-417

Forma física

Purified rabbit polyclonal IgG in buffer containing TBS with 0.01% Sodium Azide in 50% glycerol.

Almacenamiento y estabilidad

Stable for 1 year at -20°C in undiluted aliquots from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerolcontaining solutions to become frozen during storage.

Otras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 1